{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T13:32:10Z","timestamp":1784640730029,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:italic>Aedes aegypti<\/jats:italic> is a widespread mosquito in tropical and subtropical regions that causes significant mortality and morbidity in humans by transmitting diseases, such as dengue fever and Zika virus disease. Synthetic insecticides, such as pyrethroids, have been used to control <jats:italic>Ae. aegypti<\/jats:italic>, but these insecticides can also affect nontarget organisms and contaminate soil and water. This study aimed to investigate the mosquitocidal activity of <jats:italic>Pseudomonas mosselii<\/jats:italic> isolated from pond sludge against larvae of <jats:italic>Ae. aegypti<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Based on the initial results, similar time\u2010course profiles were obtained for the mosquitocidal activity of the bacterial culture and its supernatant, and the pellet resuspended in Luria\u2013Bertani (LB) medium also showed delayed toxicity. These results imply that the toxic component can be released into the medium from live bacteria. Further research indicated that the toxic component appeared in the supernatant approximately 4\u2009h after a 3\u2010mL stock was cultured in 200\u2009mL of LB medium. The stabilities of the <jats:italic>P. mosselii<\/jats:italic> culture and supernatant stored at different temperatures were also evaluated, and the best culture stability was obtained at 28\u2009\u00b0C and supernatant stability at 4\u2009\u00b0C. The bacterial culture and supernatant were toxic to larvae and pupae of not only susceptible <jats:italic>Ae. aegypti<\/jats:italic> but also pyrethroid\u2010resistant strains.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This study highlights the value of the mosquitocidal activity of <jats:italic>P. mosselii<\/jats:italic>, which has potential as an alternative insecticide to control pyrethroid\u2010resistant <jats:italic>Ae. aegypti<\/jats:italic> in the field. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.8139","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T07:34:00Z","timestamp":1713425640000},"page":"4344-4351","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>Pseudomonas mosselii<\/i>: a potential alternative for managing pyrethroid\u2010resistant <i>Aedes 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