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The bioassay results indicated that some compounds possessed notable inhibitory effects\n                      <jats:italic>in vitro<\/jats:italic>\n                      against the bacterium\n                      <jats:italic>Xanthomonas oryzae<\/jats:italic>\n                      pv.\n                      <jats:italic>oryzicola<\/jats:italic>\n                      (\n                      <jats:italic>Xoc<\/jats:italic>\n                      ). For example, compound E19 had an EC\n                      <jats:sub>50<\/jats:sub>\n                      (effective concentration for 50% activity) value of 7.1\u2009\u03bcg\/mL towards this pathogen, approximately 15\u2010 and 10\u2010fold more effective than the commercial bactericides thiodiazole copper and bismerthiazol (EC\n                      <jats:sub>50<\/jats:sub>\n                      \u2009=\u2009110.2 and 72.4\u2009\u03bcg\/mL, respectively). Subsequently, the mechanistic studies showed that compound E19 likely exerted its antibacterial efficacies by altering the cell morphology, increasing the permeability of bacterial cytoplasmic membrane, suppressing the production of bacterial extracellular polysaccharides and the extracellular enzyme activities (amylase and cellulase), and blocking the swimming motility of\n                      <jats:italic>Xoc<\/jats:italic>\n                      . Moreover, the proteomic analysis revealed that compound E19 could reduce the bacterial flagellar biosynthesis and decrease the flagellar motility by down\u2010regulating the expression of the related differential proteins.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Compound E19 exhibited good potential for further development as a bactericide candidate for control of\n                      <jats:italic>Xoc<\/jats:italic>\n                      . \u00a9 2024 Society of Chemical Industry.\n                    <\/jats:p>\n                  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