{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T23:43:12Z","timestamp":1791502992031,"version":"4.3.4"},"reference-count":48,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T00:00:00Z","timestamp":1719964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Plant pathogens cause substantial crop losses annually, posing a grave threat to global food security. Fungicides have usually been used for their control, but the rapid development of pesticide resistance renders many ineffective, therefore the search for novel and efficient green pesticides to prevent and control plant diseases has become the top priority in crop planting.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The results of bioassay studies indicated that most of the target compounds showed certain antimicrobial activity <jats:italic>in vitro<\/jats:italic>. In particular, compound <jats:bold>X7<\/jats:bold> showed high inhibitory activity against <jats:italic>Xanthomonas oryzae<\/jats:italic> pv. <jats:italic>oryzae<\/jats:italic> (<jats:italic>Xoo<\/jats:italic>), with an EC<jats:sub>50<\/jats:sub> value of 27.47\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup>, surpassing conventional control agents such as thiazole zinc (41.55\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup>) and thiodiazole copper (53.39\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup>). Further studies on molecular docking showed that <jats:bold>X7<\/jats:bold> had a strong binding affinity with 2FBW. The morphological change observed by scanning electron microscopy indicated that the surface of <jats:italic>Xoo<\/jats:italic> appears wrinkled and cracked under <jats:bold>X7<\/jats:bold> treatment and a total of 2662 proteins were identified by label\u2010free proteomic analysis. Three experiments have elucidated the mechanism whereby <jats:bold>X7<\/jats:bold> induced considerable changes in the physiological and biochemical properties of <jats:italic>Xoo<\/jats:italic>, which in turn affected the reproduction and growth of bacteria.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This work represents a pivotal advancement, offering important reference for the research and development therapeutics in combating plant pathogens. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.8266","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T02:38:53Z","timestamp":1720060733000},"page":"5388-5399","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Design, synthesis, antimicrobial activity, and mechanism of novel 3\u2010(2,4\u2010dichlorophenyl)\u2010[1,2,4]triazolo[3,4\u2010b][1,3,4]thiadiazole 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