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Quantum chemical calculations (via density functional theory) results indicated that the primary active site of Co. 6a lies in its amide structure. Mycelial morphology and physiology were investigated to elucidate the mode\u2010of\u2010action of Co. 6a, and suggested that Co. 6a produced significant cell membrane damage, accelerated electrolyte leakage, decreased succinate dehydrogenase (SDH) protein activity, and impaired physiological and biochemical functions, culminating in mycelial mortality. Molecular docking analysis revealed a robust binding energy (\u0394E\u2009=\u2009\u22127.29\u2009kcal\u2009mol<jats:sup>\u22121<\/jats:sup>) between Co. 6a and SDH. Subsequently, biosafety evaluations confirmed the environmentally\u2010friendly nature of Co. 6a via the zebrafish model, yet toxicological results indicated that Co. 6a at median lethal concentration [LC<jats:sub>50(96)<\/jats:sub>] damaged the gills, liver and intestines of zebrafish.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The above research offers a theoretical foundation for exploiting eco\u2010friendly rosin\u2010based fungicidal candidates in sustainable crop protection. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.8323","type":"journal-article","created":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T02:10:51Z","timestamp":1721441451000},"page":"5898-5908","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Design, synthesis and biological activity evaluation of eco\u2010friendly rosin\u2010based fungicides for sustainable crop 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