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RIC\u20103 (resistant to inhibitors of cholinesterase 3) has been shown to diversify by its differential mRNA splicing patterns. How such diversity influences neonicotinoid sensitivity of nAChRs of beneficial insect species remains poorly understood. We have identified a RIC\u20103 variant expressed most abundantly in the thoracic ganglia of honeybee (<jats:italic>Apis mellifera<\/jats:italic>) workers and investigated its effects on the functional expression and pharmacology of Am\u03b11\/Am\u03b18\/Am\u03b21 and Am\u03b11\/Am\u03b12\/Am\u03b18\/Am\u03b21 nAChRs expressed in <jats:italic>X. laevis<\/jats:italic> oocytes. The AmRIC\u20103 enhanced the response amplitude to the acetylcholine (ACh) of these <jats:italic>A. mellifera<\/jats:italic> nAChRs when its cRNA\u00a0was injected into oocytes at low concentrations but suppressed the ACh response amplitude at high concentrations. Co\u2010expression of the AmRIC\u20103 had a minimal impact on the affinity of ACh, but changed the efficacy of imidacloprid and clothianidin, suggesting that the presence and the level of RIC\u20103 expression can affect the nAChR responses to ACh and neonicotinoids, depending on nAChR subunit composition in honeybees. \u00a9 2024 Society of Chemical Industry.<\/jats:p>","DOI":"10.1002\/ps.8371","type":"journal-article","created":{"date-parts":[[2024,8,21]],"date-time":"2024-08-21T22:58:49Z","timestamp":1724281129000},"page":"2671-2677","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Impact of a worker bee thoracic ganglion <scp>RIC<\/scp>\u20103 variant on the actions of acetylcholine and neonicotinoids on nicotinic receptors in <scp><i>Apis 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