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In this study, we evaluated the potential of the natural compound hinokitiol against <jats:italic>S. sclerotiorum<\/jats:italic> by determining its biological activity and physiological characteristics.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Our results showed that hinokitiol strongly inhibited the hyphae expansion of <jats:italic>S. sclerotiorum<\/jats:italic>, and its effective concentration of hyphae growing inhibition by 50% (EC<jats:sub>50<\/jats:sub>) against 103\u2009<jats:italic>S. sclerotiorum<\/jats:italic> strains varied from 0.36 to 3.45\u2009\u03bcg\/mL, with an average of 1.23\u2009\u03bcg\/mL. Hinokitiol possessed better protective efficacy than therapeutic effects, and it exhibited no cross\u2010resistance between carbendazim. After treatment with hinokitiol, many vesicular protrusions developed on the mycelium with rough surface and thickened cell wall. Moreover, the cell membrane permeability and glycerol content increased, while the oxalic acid declined after hinokitiol treatment. In addition, hinokitiol induced membrane lipid peroxidation and improved the production of reactive oxygen species (ROS) in <jats:italic>S. sclerotiorum<\/jats:italic>. Importantly, real\u2010time quantitative polymerase chain reaction showed that cell wall and ROS synthesis\u2010related genes were significantly up\u2010regulated after hinokitiol treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This study revealed that hinokitiol has good biological activity against <jats:italic>S. sclerotiorum<\/jats:italic> and could be considered as an alternative bio\u2010fungicide for the resistance management in controlling sclerotinia stem rot infected by <jats:italic>S. sclerotiorum<\/jats:italic>. These investigations provided new insights into understanding the toxic action of hinokitiol against pathogenic fungi. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.8395","type":"journal-article","created":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T06:05:28Z","timestamp":1725429928000},"page":"6566-6574","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Baseline sensitivity and physiological characteristics of natural product hinokitiol against <i>Sclerotinia sclerotiorum<\/i>"],"prefix":"10.1002","volume":"80","author":[{"given":"Mengwei","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection Northwest A&amp;F University  Yangling 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