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The post\u2010transcriptional regulation of ovarian development remains unclear. Here, miR\u201031b is shown to be involved in regulating <jats:italic>Bactrocera dorsalis<\/jats:italic> ovarian development.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>CRISPR\/Cas9 was used to generate miR\u201031b loss\u2010of\u2010function mutations in <jats:italic>Bactrocera dorsalis<\/jats:italic>. The removal of miR\u201031b resulted in severely impaired ovarian development in adults, with phenotypes that included dramatically reduced egg production and hatching rates. The relationship between miR\u201031b and its target gene <jats:italic>arylsulfatase B<\/jats:italic> (<jats:italic>ARSB<\/jats:italic>) was subsequently identified using the methods of bioinformatics, transcriptomic sequencing, quantitative polymerase chain reaction (qPCR), RNA pull\u2010down and dual\u2010luciferase reporter assay. Finally, miR\u201031b was confirmed to bind the target gene <jats:italic>arylsulfatase B<\/jats:italic> to affect metabolism and thereby further hindered ovarian development of <jats:italic>Bactrocera dorsalis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Overall, these results provide new insights into molecular mechanisms at the post\u2010transcriptional level in regulating ovarian development and insect reproduction, consequently providing potential targets to control arthropod pests through the reproductive strategy. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.8513","type":"journal-article","created":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T06:45:21Z","timestamp":1730702721000},"page":"1094-1102","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The <scp>miR\u201031b<\/scp> targets <scp><i>arylsulfatase B<\/i><\/scp> 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