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The Asian citrus psyllid, <jats:italic>Diaphorina citri<\/jats:italic> Kuwayama, is the most destructive insect pest in citrus orchards because of its role as a vector for the huanglongbing pathogen, <jats:italic>Candidatus<\/jats:italic> Liberibacter asiaticus (<jats:italic>C<\/jats:italic>Las). However, specific effector proteins and their functions in <jats:italic>D.\u2009citri<\/jats:italic> remain unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>We demonstrate that <jats:italic>DcE1<\/jats:italic>, a salivary protein gene from <jats:italic>D.\u2009citri<\/jats:italic>, is predominantly expressed in the salivary gland tissues and is delivered into host plants during feeding. Transient expression in tobacco leaves revealed that <jats:italic>DcE1<\/jats:italic> was subcellularly localized in the cytoplasm and plasma membrane, where it inhibited <jats:italic>BAX<\/jats:italic>\u2010 and <jats:italic>INF1<\/jats:italic>\u2010induced cell death, suppressed callose deposition, and activated the salicylic acid pathway by upregulating the expression of endo\u2010\u03b2\u20101,3\u2010glucanase <jats:italic>NtBGL2<\/jats:italic> and regulatory protein <jats:italic>NtNPR1<\/jats:italic>. Further, <jats:italic>DcE1<\/jats:italic> knockdown by double\u2010stranded RNA (dsRNA) injection decreased the survival rates of <jats:italic>D.\u2009citri<\/jats:italic> and interrupted <jats:italic>D.\u2009citri<\/jats:italic> phloem\u2010feeding on host plants.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These results indicate that <jats:italic>DcE1<\/jats:italic> is a novel effector that promotes plant susceptibility and enables <jats:italic>D.\u2009citri<\/jats:italic> feeding. These findings enhance our understanding of <jats:italic>D.\u2009citri<\/jats:italic>\u2013plant interactions and offer a potential new target gene for the development of citrus protection strategies. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.8536","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T23:50:55Z","timestamp":1731628255000},"page":"1717-1726","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Salivary effector <scp>DcE1<\/scp> suppresses plant defense and facilitates the successful feeding of <scp>Asian<\/scp> citrus psyllid, <i>Diaphorina citri<\/i>"],"prefix":"10.1002","volume":"81","author":[{"given":"Yinhui","family":"Kuang","sequence":"first","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Pest and Disease Control of Featured Horticultural Plants\/National Navel Orange Engineering Research Center, College of 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