{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T01:44:32Z","timestamp":1778031872131,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22177043"],"award-info":[{"award-number":["22177043"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22377045"],"award-info":[{"award-number":["22377045"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["20JR5RA311"],"award-info":[{"award-number":["20JR5RA311"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Plant pathogenic bacteria and fungi had a wide impact on various crops and caused serious economic losses. Compared to traditional antibiotics and fungicides, alkaloids are noteworthy for their significant biological activities. Tetrahydrocarbazole (THCz) was widely present in many indole alkaloids and had various pharmacological activities. This study used Fischer indole synthesis method and fragment splicing strategy to design and synthesize a novel drug like chemical entity based on THCz alkaloid guidance, evaluated the antimicrobial activities of these derivatives against plant pathogenic bacteria and fungi and discussed the structure\u2013activity relationship.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among these derivatives, compound <jats:bold>KZa\u201017<\/jats:bold> displayed good antibacterial activity against <jats:italic>Xanthomonas oryzae<\/jats:italic> pv. <jats:italic>oryzae<\/jats:italic> (Xoo), <jats:italic>Xanthomonas axonopodis<\/jats:italic> pv. <jats:italic>citri<\/jats:italic>, <jats:italic>Pseudo\u2010monas sollamacearum<\/jats:italic> and <jats:italic>Erwinia aroideae<\/jats:italic>. The <jats:bold>MIC<\/jats:bold><jats:sub><jats:bold>90<\/jats:bold><\/jats:sub> values were 3.12, 1.56, 6.25, 12.5\u2009\u03bcg\/mL, respectively, which were better than the positive control drugs thiodiazole copper and bismerthiazol. The results of <jats:italic>in vivo<\/jats:italic> antibacterial activity test showed that the protective effect of compound <jats:bold>KZa\u201017<\/jats:bold> on rice at 200\u2009\u03bcg\/mL was 32.76%, which was better than that of thiodiazole copper (24.73%). The biochemical experiments showed that compound <jats:bold>KZa\u201017<\/jats:bold> could inhibit biofilm formation of Xoo, reduce drug resistance and pathogenicity of bacteria, damage cell membrane integrity, increase permeability, and cause oxidative stress damage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Most of the newly synthesized derivatives based on THCz alkaloid guidance showed favorable antimicrobial effects. In particular, compound <jats:bold>KZa\u201017<\/jats:bold> can be used as a candidate lead compound for further structural optimization and development. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.8567","type":"journal-article","created":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T03:50:10Z","timestamp":1733370610000},"page":"1752-1764","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Design, synthesis and antimicrobial activity evaluation of novel tetrahydrocarbazole derivatives against phytopathogenic fungi and bacteria"],"prefix":"10.1002","volume":"81","author":[{"given":"Yan\u2010Yan","family":"Ding","sequence":"first","affiliation":[{"name":"School of Pharmacy Lanzhou University  Lanzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian\u2010Li","family":"Dai","sequence":"additional","affiliation":[{"name":"School of Pharmacy Lanzhou University  Lanzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiong\u2010Fei","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Pharmacy Lanzhou University  Lanzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shao\u2010Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Science Huzhou University  Huzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3055-3426","authenticated-orcid":false,"given":"Zhi\u2010Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Lanzhou University  Lanzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo\u2010Liang","family":"Mou","sequence":"additional","affiliation":[{"name":"School of Pharmacy Lanzhou University  Lanzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u2010Lu","family":"Qin","sequence":"additional","affiliation":[{"name":"School of Pharmacy Lanzhou University  Lanzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Rui","family":"Jin","sequence":"additional","affiliation":[{"name":"School of Pharmacy Lanzhou University  Lanzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying\u2010Qian","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Lanzhou University  Lanzhou People's Republic of China"},{"name":"Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Science Huzhou University  Huzhou People's Republic of China"},{"name":"China State Key Laboratory of Grassland Agro\u2010ecosystems Lanzhou University  Lanzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/340247"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12436"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-10-13-0274-R"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40858-020-00355-8"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-015-0690-z"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02423-0"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph18031112"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph19084792"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2023.113955"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-021-17374-w"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115511"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101-017-9521-5"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2015.09.044"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11030493"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2015.02.059"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2010.01.159"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24101912"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.202100277"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6OB00267F"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0OB02274H"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.2174\/0115734072275884240125100455"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2108244118"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2018.11.016"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.02.062"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.521"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217-021-03929-1"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9RA05712A"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b03952"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2020.112141"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.0c07826"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5873"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.925291"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2010.04.011"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.8567","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T10:30:48Z","timestamp":1761042648000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.8567"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,5]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/ps.8567"],"URL":"https:\/\/doi.org\/10.1002\/ps.8567","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,5]]},"assertion":[{"value":"2024-07-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}