{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:47:29Z","timestamp":1790948849418,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T00:00:00Z","timestamp":1732838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32220103009"],"award-info":[{"award-number":["32220103009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>In many organisms, the Y chromosome contains important genes associated with sex determination and male reproductive development. However, there have been few studies of Y\u2010chromosome\u2010specific genes in non\u2010model species due to the incomplete information of Y chromosome genome and difficulty in sequencing. Here, we screened 90 candidate Y\u2010specific sequences in a constructed transcriptome assembly library by using the chromosome quotient method, among which 11 were unreported sequences associated with male reproductive development, including <jats:italic>Bactrocera dorsalis Y\u2010specific Oligozoospermia factor<\/jats:italic> (<jats:italic>Bdyof)<\/jats:italic> with the highest expression in the testis.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>CRISPR\/Cas9\u2010mediated knockout of <jats:italic>Bdyof<\/jats:italic> resulted in abnormal testis development, significantly reduced sperm count, and obviously lower egg hatching rate in homozygous mutant flies. In addition, <jats:italic>Bdyof<\/jats:italic> knockout decreased the expression of <jats:italic>dsx\u2010M.<\/jats:italic><\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This results provides new insights into the biological processes related to male reproductive development controlled by the Y\u2010chromosome\u2010specific gene <jats:italic>Bdyof<\/jats:italic>, thus providing a promising molecular target for the study of agricultural pests. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.8577","type":"journal-article","created":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T07:07:39Z","timestamp":1732864059000},"page":"1785-1793","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<scp><i>Bdyof<\/i><\/scp> is a Y\u2010chromosome\u2010specific gene required for male development in <scp><i>Bactrocera dorsalis<\/i><\/scp>"],"prefix":"10.1002","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4835-0018","authenticated-orcid":false,"given":"Qiuyuan","family":"Zhang","sequence":"first","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Chen","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Hubei Hongshan Laboratory, Institute of Urban and Horticultural Entomology, College of Plant Science and Technology Huazhong Agricultural University  Wuhan 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