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Therefore, we aimed to verify the insecticidal properties of <jats:italic>Bacillus amyloliquefaciens<\/jats:italic> YJNbs21.10 against aphids, isolated to determine the bioactivity of its metabolite Macrolactin A against aphids for the first time.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The results of activity tracking showed that the fermentation broth of YJNbs21.10 had the best inhibitory efficacy against <jats:italic>R. padi<\/jats:italic>, and the corrected efficiency reached 95.57% after 24\u2009h. With the continuous separation and test, the efficiency of the active components decreased: Macrolactin A, as the most active substance, had a control activity against aphids under 500\u2009mg\u2009L<jats:sup>\u22121<\/jats:sup> of 74.64% at 72\u2009h, (which was significantly lower than that of fermentation broth, indicating a synergistic effect between the active substances of each part of the strain. In addition, the result of the stereomicroscope showed that Macrolactin A damaged the body wall of aphids. The toxicity of Macrolactin A to <jats:italic>R. padi<\/jats:italic> was confirmed through the gradient test.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>In this study, <jats:italic>Bacillus amyloliquefaciens<\/jats:italic> YJNbs21.10 exhibited comparable inhibitory ability to chemical pesticides suggesting its potential to provide effective biological control on aphids. The biological activity of Macrolactin A against aphids was also verified for the first time, in this experiment, the EC<jats:sub>50<\/jats:sub> of this substance against aphids was 169.02\u2009mg\u2009L<jats:sup>\u22121<\/jats:sup> (24\u2009h), which provided strong evidence that YJNbs21.10 may act as an effective agent for the prevention of aphid. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.8589","type":"journal-article","created":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T05:34:55Z","timestamp":1733463295000},"page":"1882-1893","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Isolation of Macrolactin A from a new <scp><i>Bacillus amyloliquefaciens<\/i><\/scp> and its aphicidal activity against <scp><i>Rhopalosiphum 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