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A flavin\u2010containing monooxygenase gene (<jats:italic>PxFMO2<\/jats:italic>) from <jats:italic>Plutella xylostella<\/jats:italic> and a P450 gene (<jats:italic>SeCYP9A186<\/jats:italic>, F116V mutant allele) from <jats:italic>Spodoptera exigua<\/jats:italic> have been reported to be involved in insecticide resistance. In this study, we aim to utilize transgenic technology to validate their <jats:italic>in vivo<\/jats:italic> detoxification functions in <jats:italic>Plutella xylostella<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>We established two transgenic strains of <jats:italic>Plutella xylostella<\/jats:italic> with expressing an endogenous <jats:italic>PxFMO2<\/jats:italic> gene from <jats:italic>Plutella xylostella<\/jats:italic> and an exogenous <jats:italic>SeCYP9A186<\/jats:italic> gene from <jats:italic>S. exigua<\/jats:italic>, respectively. Bioassays demonstrated that the transgenic <jats:italic>Plutella xylostella<\/jats:italic> strain (IPP\u2010FMO2) expressing <jats:italic>PxFMO2<\/jats:italic> exhibited a 12\u2010fold resistance to emamectin benzoate and a 6.4\u2010fold resistance to chlorantraniliprole compared to the background strain (IPP\u2010S). In contrast, the transgenic <jats:italic>Plutella xylostella<\/jats:italic> strain (IPP\u20109A186) expressing <jats:italic>SeCYP9A186<\/jats:italic> displayed a 235\u2010fold resistance to emamectin benzoate and a 115\u2010fold resistance to abamectin. Moreover, resistance to emamectin benzoate in the IPP\u20109A186 strain of <jats:italic>Plutella xylostella<\/jats:italic> was inherited as an incompletely dominant trait and was genetically linked to the transgene locus.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Our results not only elucidated the <jats:italic>in vivo<\/jats:italic> contribution of the <jats:italic>PxFMO2<\/jats:italic> and <jats:italic>SeCYP9A186<\/jats:italic> to the insecticide resistance phenotype in <jats:italic>Plutella xylostella<\/jats:italic>, but also provided a genetic engineering toolkit to manipulate resistance pathways. These insights and methodologies could further aid in developing sustainable pest management strategies in <jats:italic>Plutella xylostella<\/jats:italic>. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.8598","type":"journal-article","created":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T08:56:41Z","timestamp":1733734601000},"page":"2000-2008","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Transgenic expression of <scp><i>SeCYP9A186<\/i><\/scp> and <scp><i>PxFMO2<\/i><\/scp> confers resistance to emamectin benzoate in <scp><i>Plutella xylostella<\/i><\/scp>"],"prefix":"10.1002","volume":"81","author":[{"given":"Falong","family":"Wang","sequence":"first","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University  Nanjing 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