{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T14:21:33Z","timestamp":1791814893396,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The function of some testis\u2010specific genes (TSGs) in model insects have been studied, but their function in non\u2010model insects remains largely unexplored. In the present study, we identified several TSGs in the fall armyworm (FAW), a significant agricultural pest, through comparative transcriptomic analysis. A testis\u2010specific gene cluster (TSGC) comprising multiple functional genes and long non\u2010coding RNAs was found.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Quantitative PCR analysis validated the testis\u2010specific expression of genes within this cluster across different developmental stages. Interspecies collinearity analysis indicated that this TSGC is conserved among lepidopterans. To elucidate the function of this TSGC, we employed the CRISPR\/Cas9 gene\u2010editing technique to generate loss\u2010of\u2010function mutants. Notably, the hatching rate of eggs produced by wild\u2010type females mated with mutant males (approximately 30%) was substantially lower than that of eggs from wild\u2010type females mated with wild\u2010type males (approximately 80%). Additionally, a significant reduction in the size and number of sperm was observed in the testis of mutant males compared to wild\u2010type males.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Collectively, this study not only elucidates the role of the TSGC in supporting male fertility in the FAW, which provides valuable information for understanding the mechanism of male reproduction in insects, but also identifies potential target genes for the development of genetic control strategies against lepidopteran pests. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ps.8634","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T04:40:17Z","timestamp":1736224817000},"page":"2355-2363","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Knockout of a testis\u2010specific gene cluster impairs male fertility in the fall armyworm, <i>Spodoptera frugiperda<\/i>"],"prefix":"10.1002","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9275-0831","authenticated-orcid":false,"given":"Pei","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Protection Resources and Pest Management of the Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection Northwest A&amp;F University  Yangling 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