{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T06:23:10Z","timestamp":1789107790211,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003995","name":"Natural Science Foundation of Anhui Province","doi-asserted-by":"publisher","award":["2108085QB76"],"award-info":[{"award-number":["2108085QB76"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2025,6]]},"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n Development of novel chiral antifungal agents for effective control of plant pathogens is urgently needed. In this study, a series of pyrazol\u20105\u2010yl\u2010benzamide derivatives containing chiral oxazoline moiety were rationally designed and developed based on molecular docking.<\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n The\n in vitro<\/jats:italic>\n antifungal assay results indicated that compounds (\n rac<\/jats:italic>\n )\u2010\n 4h<\/jats:bold>\n (R\n 1<\/jats:sup>\n \u2009=\u2009Et), (\n S<\/jats:italic>\n )\u2010\n 4\u2009h<\/jats:bold>\n (R\n 1<\/jats:sup>\n \u2009=\u2009\n S<\/jats:italic>\n \u2010Et) and (\n R<\/jats:italic>\n )\u2010\n 4\u2009h<\/jats:bold>\n (R\n 1<\/jats:sup>\n \u2009=\u2009\n R<\/jats:italic>\n \u2010Et) exhibited remarkable antifungal activities against\n Valsa mali<\/jats:italic>\n with median effective concentration (EC\n 50<\/jats:sub>\n ) values of 0.24, 0.06 and 1.08\u2009mg\/L, respectively. Preliminary structure\u2013activity relationships (SARs) revealed that the modification of the chiral substituent group at the oxazoline moiety significantly affected the antifungal activities of the target compounds. Furthermore, compounds (\n S<\/jats:italic>\n )\u2010\n 4h<\/jats:bold>\n (87.5%) and (\n R<\/jats:italic>\n )\u2010\n 4h<\/jats:bold>\n (84.3%) exhibited\n in vivo<\/jats:italic>\n protective activities comparable to tebuconazole (87.5%) against\n V. mali<\/jats:italic>\n . Subsequent molecular docking analysis, succinate dehydrogenase (SDH) enzyme inhibition assays and molecular dynamic (MD) simulations verified that the potential target enzyme of this class of derivatives could be SDH and helped to explain the large difference in antifungal activities of compounds (\n S<\/jats:italic>\n )\u2010\n 4h<\/jats:bold>\n and (\n R<\/jats:italic>\n )\u2010\n 4h<\/jats:bold>\n . Confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) observations confirmed that these two compounds severely disrupted the mycelial morphology of\n V. mali<\/jats:italic>\n . Theoretical calculation studies provided some insight into the subsequent modification of such pyrazol\u20105\u2010yl\u2010benzamide derivatives. Resistance frequency studies showed that (\n S<\/jats:italic>\n )\u2010\n 4h<\/jats:bold>\n and (\n R<\/jats:italic>\n )\u2010\n 4h<\/jats:bold>\n treatments were less likely to produce resistant fungal strains than tebuconazole. Meanwhile, compounds (\n S<\/jats:italic>\n )\u2010\n 4h<\/jats:bold>\n and (\n R<\/jats:italic>\n )\u2010\n 4h<\/jats:bold>\n exhibited no apparent toxicity to the\n Apis mellifera<\/jats:italic>\n L. population.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n Therefore, these derivatives are potential candidates for the development of novel chiral fungicides for crop protection. \u00a9 2025 Society of Chemical Industry.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.8663","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T04:27:06Z","timestamp":1737001626000},"page":"2922-2934","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Design and development of pyrazol\u20105\u2010ylbenzamide derivatives containing chiral oxazoline moiety as fungicides based on molecular docking"],"prefix":"10.1002","volume":"81","author":[{"given":"Xiang","family":"Cheng","sequence":"first","affiliation":[{"name":"College of Materials and Chemistry and School of Plant Protection Anhui Agricultural University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Materials and Chemistry and School of Plant Protection Anhui Agricultural University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanjian","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Materials and Chemistry and School of Plant Protection Anhui Agricultural University Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanglei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Materials and Chemistry and School of Plant Protection Anhui Agricultural University Hefei 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