{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:32:00Z","timestamp":1790839920617,"version":"4.1.0"},"reference-count":90,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T00:00:00Z","timestamp":1738540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T00:00:00Z","timestamp":1738540800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Rice blast, caused by\n                      <jats:italic>Pyricularia oryzae<\/jats:italic>\n                      , is a devastating fungal disease threatening global rice production. Overreliance on chemical fungicides has raised environmental concerns and led to resistant strains, necessitating the development of sustainable alternatives. This study integrated marine microbiology and natural antifungal compounds to create eco\u2010friendly alternatives to chemical fungicides for disease management.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      We identified\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      R64 with broad\u2010spectrum antimicrobial activity from mangrove soil in the Mai Po Nature Reserve. The R64 fermentation extract (RFE) exhibited multifaceted inhibition of\n                      <jats:italic>P.\u2009oryzae<\/jats:italic>\n                      , suppressing mycelial growth, conidiation, conidial germination and appressorial formation, while disturbing cell wall and membrane function. It also attenuated virulence by impairing appressorial penetration and invasive growth. Further chemical analysis identified phenazines and quinolines as the primary compounds in RFE, corroborated by PCR detection of corresponding phenazine biosynthetic gene clusters. Comparative bioassays with two main bioactive components of RFE, phenazine\u20101\u2010carboxamide (PCN) and phenazine\u20101\u2010carboxylic acid (PCA), against\n                      <jats:italic>P.\u2009oryzae<\/jats:italic>\n                      implicated PCN as the principal antifungal effector. RFE and PCN had higher efficacy than tricyclazole in\n                      <jats:italic>P.\u2009oryzae<\/jats:italic>\n                      growth inhibition, but were less effective than isoprothiolane. Furthermore, RFE and PCN displayed lower acute ecotoxicity to an environmental indicator organism than isoprothiolane, suggesting their potential as sustainable biopesticides for rice blast management.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Natural products from mangrove soil bacterium\n                      <jats:italic>P.\u2009aeruginosa<\/jats:italic>\n                      R64 inhibited key developmental and infection processes of\n                      <jats:italic>P.\u2009oryzae<\/jats:italic>\n                      , effectively reducing rice blast development. The promising disease inhibition and low ecotoxicity of mangrove\u2010associated bacteria highlight their untapped potential for innovative, eco\u2010friendly fungicide mining for sustainable agriculture. \u00a9 2025 The Author(s).\n                      <jats:italic>Pest Management Science<\/jats:italic>\n                      published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.8684","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T05:06:56Z","timestamp":1738559216000},"page":"3122-3135","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Investigation of the mechanisms involved in the biocontrol activities of natural products from a marine soil bacterium against rice 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