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Successful development of the ovary is dependent on vitellogenin (Vg) transport into oocytes via the vitellogenin receptor (VgR). Exploring the\n                      <jats:italic>VgR<\/jats:italic>\n                      function is crucial for understanding the physiological mechanisms of insect ovarian development. However, the functional role of VgR in\n                      <jats:italic>Bactrocera dorsalis<\/jats:italic>\n                      (Hendel), a notorious agricultural invasive pest with exceptional reproductive plasticity, remains unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Here, we identified\n                      <jats:italic>BdVgR<\/jats:italic>\n                      , an ovary\u2010specific receptor with 1903 amino acids, as a critical determinant of reproductive success. CRISPR\/Cas9\u2010mediated\n                      <jats:italic>BdVgR<\/jats:italic>\n                      knockout resulted in a 211\u2010bp genomic deletion spanning exonic (126\u2009bp) and intronic (85\u2009bp) regions, leading to near\u2010complete loss of\n                      <jats:italic>VgR<\/jats:italic>\n                      expression in female adults. Functional analyses revealed that\n                      <jats:italic>BdVgR<\/jats:italic>\n                      deficiency disrupted ovarian Vg (Vg1\/Vg2\/Vg3) accumulation, impaired ovary maturation, and thus caused severe reproductive defects, including a decrease in the size of the ovaries by 49%, mating rates by 45%, egg production by 38%, and hatching rate by 22%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Collectively, these findings indicate that\n                      <jats:italic>BdVgR<\/jats:italic>\n                      plays a key role in the reproductive process in\n                      <jats:italic>B<\/jats:italic>\n                      .\n                      <jats:italic>dorsalis<\/jats:italic>\n                      , and that disrupting VgR function can inhibit egg production, leading to sterility, which highlights the potential that targeting VgR via CRISPR can create genetically sterile females. Data are discussed with regard to integration of a sterile insect technique approach in the design of novel, efficient and safe pest management tactics. \u00a9 2025 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.8857","type":"journal-article","created":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T06:14:37Z","timestamp":1745993677000},"page":"5043-5051","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<scp>CRISPR<\/scp>\n                    \/Cas9\u2010mediated vitellogenin receptor knockout impairs vitellogenin uptake and reproduction in\n                    <i>Bactrocera dorsalis<\/i>"],"prefix":"10.1002","volume":"81","author":[{"given":"Qian\u2010Ping","family":"Xie","sequence":"first","affiliation":[{"name":"Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection, Southwest University  Chongqing 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Ghent University in Belgium Southwest University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9678-5425","authenticated-orcid":false,"given":"Wei","family":"Dou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection, Southwest University  Chongqing China"},{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Academy of Agricultural Sciences, Southwest University  Chongqing China"},{"name":"International China\u2010Belgium Joint Laboratory on Sustainable Crop Pest Control between Southwest University in China and Ghent University in Belgium Southwest University  Chongqing 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