{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T19:25:51Z","timestamp":1791228351656,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T00:00:00Z","timestamp":1747267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T00:00:00Z","timestamp":1747267200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272527"],"award-info":[{"award-number":["32272527"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2600100"],"award-info":[{"award-number":["2021YFC2600100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013314","name":"Higher Education Discipline Innovation Project","doi-asserted-by":"publisher","award":["B18044"],"award-info":[{"award-number":["B18044"]}],"id":[{"id":"10.13039\/501100013314","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2025,9]]},"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Insect spermatogenesis is a complex process. Numerous genes are involved in sperm motility, which is crucial for male fertility. Few long non\u2010coding RNAs (lncRNAs) in the testis regulate insect spermatogenesis. We previously identified 364 testis\u2010enriched lncRNAs in the globally invasive pest\n Zeugodacus cucurbitae<\/jats:italic>\n Coquillett. One of these lncRNAs,\n lnc94641<\/jats:italic>\n , is abundantly expressed in the testis; however, its role in spermatogenesis remains unknown.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n Suppression of\n lnc94641<\/jats:italic>\n expression led to a 60% decrease in spermatozoa count and a 29% decrease in offspring hatchability. A microRNA (miRNA), miR\u2010957\u20103p, was experimentally demonstrated to bind to\n lnc94641<\/jats:italic>\n competitively. miR\u2010957\u20103p overexpression recapitulated reproductive defect phenotypes similar to those caused by\n lnc94641<\/jats:italic>\n knockdown. Furthermore, target gene predictions combined with quantitative reverse transcription PCR, RNA pull\u2010down, and dual luciferase reporter assays confirmed that miR\u2010957\u20103p targets\n voltage\u2010gated potassium channel 5<\/jats:italic>\n (\n VGKC5<\/jats:italic>\n ) and\n odorant receptor 85c<\/jats:italic>\n (\n OR85c<\/jats:italic>\n ), elucidating a functional lncRNA\u2013miRNA\u2013mRNA competing endogenous RNA (ceRNA) regulatory axis. Fluorescence\n in situ<\/jats:italic>\n hybridization (FISH) assays demonstrated the co\u2010localization of\n lnc94641<\/jats:italic>\n , miR\u2010957\u20103p, and\n VGKC5<\/jats:italic>\n \/\n OR85c<\/jats:italic>\n in the mature and transformed regions of the testes. Suppression of\n VGKC5<\/jats:italic>\n \/\n OR85c<\/jats:italic>\n expression resulted in a 68% and 50% decrease in spermatozoa number and an 18% and 21% decrease in offspring hatchability, mirroring the phenotype observed with\n lnc94641<\/jats:italic>\n \u2010silencing, thereby reinforcing the mechanistic coherence of this regulatory network.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n These results revealed a ceRNA axis mediated by \u2018\n lnc94641<\/jats:italic>\n \u2013miR957\u20103p\u2013\n VGKC5\/OR85c<\/jats:italic>\n \u2019 involved in spermatogenesis that impairs male fertility in the melon fly. Molecular perturbations (lncRNA knockdown or miRNA overexpression) consistently impair sperm production and offspring viability by dysregulating ion channels and chemosensory genes. This mechanistically resolved pathway, centered on the core components\n VGKC5<\/jats:italic>\n and\n OR85c<\/jats:italic>\n , revealed conserved reproductive vulnerabilities that could enable the targeted genetic control of this agricultural pest. \u00a9 2025 Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/ps.8874","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T06:01:36Z","timestamp":1747288896000},"page":"5201-5213","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The competing endogenous\n RNA<\/scp>\n lnc94641<\/i>\n \u2013\n miR957<\/scp>\n \u20103p mediates male fertility in\n \n Zeugodacus cucurbitae<\/i>\n <\/scp>\n Coquillett"],"prefix":"10.1002","volume":"81","author":[{"given":"Dong","family":"Chen","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection, Southwest University Chongqing China"},{"name":"International Joint Laboratory of China\u2010Belgium on Sustainable Crop Pest Control Academy of Agricultural Sciences, Southwest University Chongqing China"},{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi\u2010Tong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection, Southwest University Chongqing China"},{"name":"International Joint Laboratory of China\u2010Belgium on Sustainable Crop Pest Control Academy of Agricultural Sciences, Southwest University Chongqing China"},{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection, Southwest University Chongqing China"},{"name":"International Joint Laboratory of China\u2010Belgium on Sustainable Crop Pest Control Academy of Agricultural Sciences, Southwest University Chongqing China"},{"name":"School of Agricultural Sciences, Jiangxi Agricultural University Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Liang","family":"Han","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection, Southwest University Chongqing China"},{"name":"International Joint Laboratory of China\u2010Belgium on Sustainable Crop Pest Control Academy of Agricultural Sciences, Southwest University Chongqing China"},{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8334-3313","authenticated-orcid":false,"given":"Guy","family":"Smagghe","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection, Southwest University Chongqing China"},{"name":"International Joint Laboratory of China\u2010Belgium on Sustainable Crop Pest Control Academy of Agricultural Sciences, Southwest University Chongqing China"},{"name":"Department of Plants and Crops, Faculty of Bioscience Engineering Ghent University Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1247-3302","authenticated-orcid":false,"given":"Ying","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Insect Biotechnology in Plant Protection Institute for Insect Biotechnology, Justus\u2010Liebig\u2010University Giessen Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7314-7391","authenticated-orcid":false,"given":"Hong\u2010Bo","family":"Jiang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection, Southwest University Chongqing China"},{"name":"International Joint Laboratory of China\u2010Belgium on Sustainable Crop Pest Control Academy of Agricultural Sciences, Southwest University Chongqing China"},{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8777-5268","authenticated-orcid":false,"given":"Jin\u2010Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection, Southwest University Chongqing China"},{"name":"International Joint Laboratory of China\u2010Belgium on Sustainable Crop Pest Control Academy of Agricultural Sciences, Southwest University Chongqing China"},{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3625-4358","authenticated-orcid":false,"given":"Dong","family":"Wei","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection, Southwest University Chongqing China"},{"name":"International Joint Laboratory of China\u2010Belgium on Sustainable Crop Pest Control Academy of Agricultural Sciences, Southwest University Chongqing China"},{"name":"Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05547-7"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.230136"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1127\/entomologia\/2023\/1988"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects12070638"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2095-3119(18)61976-8"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2013.10.009"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1953-y"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.199547.115"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12729"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jia.2022.11.003"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2021.02.003"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jen.13044"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.4137\/IJIS.S24582"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.117.040170"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41597-020-0387-9"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1127\/entomologia\/2021\/1037"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1127\/entomologia\/2023\/1845"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2012.08.006"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1127\/entomologia\/2022\/1363"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1127\/entomologia\/2024\/2771"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8044"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp033"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm995"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng2135"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl243"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-2-r19"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147147"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1507-3"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep14642"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00566-8"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2024.103315"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-024-06961-5"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.885767"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.13082"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008771"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks943"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0078915"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkab080"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.13383"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12986"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3606"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.09.028"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2020.103413"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2024.105811"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.823403"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242015399"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2022.104244"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/164_2021_465"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2116887119"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3635"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2013.0094"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-020-03363-x"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2073-7"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2015.00073"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01507"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241411277"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00028.2010"},{"key":"e_1_2_10_59_1","first-page":"819","article-title":"T\u2010type Ca2+ channels in spermatogenic cells and sperm","volume":"466","author":"Darszon A","year":"2014","journal-title":"Philos Trans R Soc Lond B Biol Sci"}],"container-title":["Pest Management Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.8874","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ps.8874","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ps.8874","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T19:54:47Z","timestamp":1783022087000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/ps.8874"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,15]]},"references-count":58,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1002\/ps.8874"],"URL":"https:\/\/doi.org\/10.1002\/ps.8874","archive":["Portico"],"relation":{},"ISSN":["1526-498X","1526-4998"],"issn-type":[{"value":"1526-498X","type":"print"},{"value":"1526-4998","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,15]]},"assertion":[{"value":"2024-12-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}