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Among them, compound\n                      <jats:bold>6d<\/jats:bold>\n                      exhibited the best antifungal activity against\n                      <jats:italic>B. dothidea<\/jats:italic>\n                      , with EC\n                      <jats:sub>50<\/jats:sub>\n                      value of 0.052\u2009mg\/L, which was significantly stronger than carbendazim (EC\n                      <jats:sub>50<\/jats:sub>\n                      \u2009=\u20090.40\u2009mg\/L).\n                      <jats:italic>In vivo<\/jats:italic>\n                      antifungal activity assay on apple fruits and branches showed that\n                      <jats:bold>6d<\/jats:bold>\n                      displayed significant protective and curative effects, both superior to carbendazim. In the preliminary antifungal mechanism study,\n                      <jats:bold>6d<\/jats:bold>\n                      can damage the surface morphology of mycelia, destroy the integrity and permeability of cell membrane, increase intracellular ROS content, thereby inhibiting the growth of\n                      <jats:italic>B. dothidea<\/jats:italic>\n                      . Compound\n                      <jats:bold>6d<\/jats:bold>\n                      also significantly inhibited laccase with IC\n                      <jats:sub>50<\/jats:sub>\n                      value of 8.45\u2009\u03bcM, much stronger than cysteine and PMDD\u20105Y. Molecular docking also confirmed the binding affinity and interaction mode of\n                      <jats:bold>6d<\/jats:bold>\n                      with laccase.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      This study presented a promising lead compound for the study on novel laccase inhibitors as fungicidal agrochemicals, which demonstrated significant anti\u2010\n                      <jats:italic>B. dothidea<\/jats:italic>\n                      activity and laccase inhibitory activity. \u00a9 2025 Society of Chemical Industry.\n                    <\/jats:p>\n                  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