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Blood\u2010feeding female mosquitoes not only sustain their reproduction, but also facilitate pathogen dissemination. Therefore, studying female mosquito reproduction and targeting genes or proteins related to reproduction could lead to an alternative method of vector control. Herein, we focused on the role of MD\u20102\u2010related lipid\u2010recognition protein\u2010like (ML) in the reproduction of female mosquitoes [\n                      <jats:italic>Culex pipiens pallens<\/jats:italic>\n                      (Diptera: Culicidae)].\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      <jats:italic>CpML<\/jats:italic>\n                      was predominantly expressed in female mosquito ovaries. Injection of a small interfering RNA targeting\n                      <jats:italic>CpML<\/jats:italic>\n                      resulted in reduced ovary size, and a decrease in the number of follicles and egg rafts. Knocking down\n                      <jats:italic>CpML<\/jats:italic>\n                      disrupted lipid metabolism by increasing triglyceride levels in ovaries. Knockdown of\n                      <jats:italic>CpML<\/jats:italic>\n                      resulted in a decrease in\n                      <jats:italic>CpVg<\/jats:italic>\n                      expression in the ovaries, which in turn increased the expression of\n                      <jats:italic>CpDGAT1<\/jats:italic>\n                      within the ovaries, ultimately leading to an increase in triglyceride content.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Our research found that knocking down\n                      <jats:italic>CpML<\/jats:italic>\n                      impaired reproduction in\n                      <jats:italic>Culex<\/jats:italic>\n                      mosquitoes by increasing triglyceride levels in the ovaries. This study presents strong evidence that\n                      <jats:italic>CpML<\/jats:italic>\n                      is vital for female reproductive success, serving a key role in regulating lipid metabolism in\n                      <jats:italic>C.\u2009p.\u2009pallens<\/jats:italic>\n                      , and thereby enhancing our understanding of insect development and reproduction. \u00a9 2025 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.8896","type":"journal-article","created":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T05:50:22Z","timestamp":1748584222000},"page":"5428-5438","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>MD<\/scp>\n                    \u20102\u2010related lipid\u2010recognition protein\u2010like is involved in mosquito reproduction by affecting lipid 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