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However, the involvement of mechanosensory perception in oviposition behavior of tephritid flies remains largely unexplored. Given that frugivorous tephritid flies use extraordinarily sharp\u2010tipped ovipositors to probe the stiff fruit peel before laying eggs, we speculated that mechanosensory\u2010responsive pathways mediate the oviposition behavior.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      In this study, we combined oviposition assays and RNA interference (RNAi) techniques to investigate how mechanosensory pathways mediate oviposition behaviors in the notorious invasive tephritid fly,\n                      <jats:italic>Bactrocera dorsalis<\/jats:italic>\n                      . The results show that both substrate stiffness and ovarian distention influenced the oviposition decision of female flies. Furthermore, silencing the\n                      <jats:italic>BdTMC<\/jats:italic>\n                      gene impaired the ability of flies to discriminate substrate hardness, while treatment with\n                      <jats:italic>dsBdNomPC<\/jats:italic>\n                      disrupted the internal pressure balance, leading to the accumulation of eggs in the ovary. Notably, a neuropeptide,\n                      <jats:italic>BdNPLP1<\/jats:italic>\n                      was found to be involved in mechanosensitive signal transduction downstream of\n                      <jats:italic>BdTMC<\/jats:italic>\n                      and\n                      <jats:italic>BdNomPC<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>\n                      Overall, our findings decipher the molecular mechanism underlying mechanosensory pathways mediating egg\u2010laying behavior in\n                      <jats:italic>B. dorsalis<\/jats:italic>\n                      . We propose that oviposition decision\u2010making is driven by a combination of internal and external mechanosensitive inputs, which offer new insights into the complex system of insect egg\u2010laying regulation. \u00a9 2025 Society of Chemical Industry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.8908","type":"journal-article","created":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T05:20:22Z","timestamp":1747113622000},"page":"5551-5560","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Multiple mechanosensory pathways mediate oviposition behavior in\n                    <scp>\n                      <i>Bactrocera dorsalis<\/i>\n                    <\/scp>\n                    (Diptera: Tephritidae)"],"prefix":"10.1002","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8328-4197","authenticated-orcid":false,"given":"Guijian","family":"Zhang","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuang","family":"Wu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science and Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Cui","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Insect Resource Application and Sustainable Pest Control, College of Plant Science and Technology Huazhong Agricultural University  Wuhan 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