{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T21:10:40Z","timestamp":1784409040407,"version":"3.55.0"},"reference-count":65,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pest Management Science"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Grapholitha molesta<\/jats:italic>\n                      and\n                      <jats:italic>Carposina sasakii<\/jats:italic>\n                      are notorious pests that damage fruits, and are ranked among the most high\u2010priority control targets in orchards. Chronic mixed infestation of these two economically destructive species not only pose formidable challenges to integrated pest management efforts, but also have substantial impacts on global fruit trade. Therefore, a non\u2010destructive, rapid and efficient identification technology is urgently needed. In comparison with conventional detection methods, the environmental DNA (eDNA) approach does not require direct contact or capture of organisms and can monitor multiple species simultaneously. In this study, an innovative eDNA\u2010based methodology was developed and optimized for precise detection of\n                      <jats:italic>G. molesta<\/jats:italic>\n                      and\n                      <jats:italic>Carposina sasakii<\/jats:italic>\n                      , and was finally applied to assess their infestation levels across different orchards.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>The results demonstrated that eDNA with the highest concentration and quality was obtained through a filtration process combining a 10\u2009\u03bcm mixed cellulose ester (MCE) membrane and a 0.45\u2009\u03bcm cellulose acetate (CA) membrane, after soaking the victim pulp for 15\u2009min. The primers developed for these two species of fruit moths exhibited high sensitivity, with minimum detection limits of 0.1 and 0.01\u2009ng\/ul, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>The eDNA\u2010based technology successfully detected the target moth species in orchards, regardless of whether the fruit was damaged by one or more moths. This study provided a critical theoretical foundation for the development of non\u2010destructive fruit pest testing technology, and also offered valuable references for eDNA research on other species, which will be vital to the field monitoring of fruit\u2010boring moths. \u00a9 2025 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ps.8950","type":"journal-article","created":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:54:24Z","timestamp":1748912064000},"page":"5973-5984","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Detection of\n                    <i>Grapholitha molesta<\/i>\n                    and\n                    <i>Carposina sasakii<\/i>\n                    based on environmental\n                    <scp>DNA<\/scp>\n                    and its field application"],"prefix":"10.1002","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2852-4221","authenticated-orcid":false,"given":"Anjuan","family":"Li","sequence":"first","affiliation":[{"name":"College of Plant Protection China Agricultural University  Beijing China"},{"name":"Key Laboratory of Surveillance and Management for Plant Quarantine Pests Ministry of Agriculture and Rural Affairs  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1281-2108","authenticated-orcid":false,"given":"Zhihong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Plant Protection China Agricultural University  Beijing China"},{"name":"Key Laboratory of Surveillance and Management for Plant Quarantine Pests Ministry of Agriculture and Rural Affairs  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7312-896X","authenticated-orcid":false,"given":"Lijun","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Plant Protection China Agricultural University  Beijing China"},{"name":"Key Laboratory of Surveillance and Management for Plant Quarantine Pests Ministry of Agriculture and Rural Affairs  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1509","article-title":"Progress in the application of IPM to control the oriental fruit moth (Grapholitha molesta) in China","volume":"50","author":"Fan RJ","year":"2013","journal-title":"J Appl Entomol"},{"key":"e_1_2_9_3_1","first-page":"36","article-title":"Study on occurrence of Carposina niponensis in orchards","volume":"5","author":"Chen C","year":"2015","journal-title":"J 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