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The fatty acid portion was methylated and analyzed by GC\/MS, ESI Q\u2010TOF MS and<jats:sup>1<\/jats:sup>H\u2010NMR. The peptide portion, of which the amino acid composition was obtained by HPLC combined with a phenyl isothiocyanate (PITC) derivatization methods, was analyzed by ESI Q\u2010TOF MS. Comparing the obtained results with surfactin C<jats:sub>13<\/jats:sub>showed that the new lipopeptide has a peptide moiety similar to that of surfactin and the difference exists in the fatty acid portion, which is an iso\u2010C<jats:sub>12<\/jats:sub>\u03b2\u2010hydroxy fatty acid. The critical micelle concentration (CMC) of this new homologue is estimated to be 6.27 \u00d7 10<jats:sup>\u22125<\/jats:sup>mol\/l in 10 mmol\/l phosphate buffer solution (PBS, pH 8.0) at 30 \u00b0C, and the surface tension at CMC (\u03b3<jats:sub>CMC<\/jats:sub>) achieved is as little as 27.71 mN\/m. The hemolytic activities of the C<jats:sub>12<\/jats:sub>\u2010lipopeptide on 2% human erythrocytes showed a HC<jats:sub>50<\/jats:sub>of 26.5 \u00b5mol\/l. Copyright \u00a9 2008 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.1017","type":"journal-article","created":{"date-parts":[[2008,2,11]],"date-time":"2008-02-11T11:20:18Z","timestamp":1202728818000},"page":"864-875","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Isolation and characterization of a C<sub>12<\/sub>\u2010lipopeptide produced by<i>Bacillus subtilis<\/i>HSO 121"],"prefix":"10.1002","volume":"14","author":[{"given":"Xiang\u2010Yang","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shi\u2010Zhong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo\u2010Zhong","family":"Mu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,2,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2006.08.001"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2006.10.004"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2004.01.010"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2004.04.006"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.2174\/138620703106298716"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(68)90503-2"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530051432"},{"key":"e_1_2_1_9_2","first-page":"153","article-title":"Surfactin sodium salt: an excellent biosurfactant for cosmetics","volume":"52","author":"Yoneda T","year":"2001","journal-title":"J. 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