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HPLC\u2013electrospray ionization mass spectrometry analysis on pig parotid secretory granule extracts evidenced a peptide with a molecular mass value of 5381.1 \u00b1 0.6 Da and its truncated form, devoid of the <jats:italic>C<\/jats:italic>\u2010terminal Ala residue, with a molecular mass value of 5310.1 \u00b1 0.6 Da. The complete sequence of pig statherin gene was determined by sequencing the full\u2010length cDNA obtained by rapid amplification of cDNA ends. The gene is 549 base pairs long and contains an open reading frame of 185 nucleotides, encoding a 42\u2010amino acid secretory polypeptide with a signal peptide of 19 residues. This sequence presents some typical features of the four statherins characterized till now, showing the highest degree of amino acid identity with bovine (57%) and human statherin (39%). Pig statherin is mono\u2010phoshorylated on Ser\u20103, while primate statherins already characterized are di\u2010phosphorylated on Ser\u20102 and Ser\u20103. This difference, probably connected to the Asp\u20104 \u2192 Glu substitution, suggests the involvement of the Golgi\u2010casein kinase, which strictly recognizes the SX(E\/pS) consensus sequence. Copyright \u00a9 2010 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.1232","type":"journal-article","created":{"date-parts":[[2010,5,7]],"date-time":"2010-05-07T19:26:41Z","timestamp":1273260401000},"page":"269-275","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Structural characterization of a new statherin from pig parotid granules"],"prefix":"10.1002","volume":"16","author":[{"given":"Barbara","family":"Manconi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Fanali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiziana","family":"Cabras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosanna","family":"Inzitari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Patamia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuele","family":"Scarano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonella","family":"Fiorita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Vitali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Castagnola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Messana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Teresa Sanna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,5,7]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"5968","DOI":"10.1016\/S0021-9258(18)42650-6","article-title":"Salivary statherin. 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