{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:06:22Z","timestamp":1788350782278,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2010,5,27]],"date-time":"2010-05-27T00:00:00Z","timestamp":1274918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Huntington's disease (HD) is a neurodegenerative disorder that affects approximately 1 in 10 000 individuals. The underlying gene mutation was identified as a CAG\u2010triplet repeat expansion in the gene <jats:italic>huntingtin<\/jats:italic>. The CAG sequence codes for glutamine, and in HD, an expansion of the polyglutamine (poly\u2010Q) stretch above 35 glutamine residues results in pathogenicity. It has been demonstrated in various animal models that only the expression of exon 1 huntingtin, a 67\u2010amino acid\u2010long polypeptide plus a variable poly\u2010Q stretch, is sufficient to cause full HD\u2010like pathology. Therefore, a deeper understanding of exon 1 huntingtin, its structure, aggregation mechanism and interaction with other proteins is crucial for a better understanding of the disease. Here, we describe the synthesis of a 109\u2010amino acid\u2010long exon 1 huntingtin peptide including a poly\u2010Q stretch of 42 glutamines. This microwave\u2010assisted solid phase peptide synthesis resulted in milligram amounts of peptide with high purity. We also synthesized a nonpathogenic version of exon 1 huntingtin (90\u2010amino acid long including a poly\u2010Q stretch of 23 glutamine residues) using the same strategy. In circular dichroism spectroscopy, both polypeptides showed weak alpha\u2010helical properties with the longer peptide showing a higher helical degree. These model peptides have great potential for further biomedical analyses, e.g. for large\u2010scale pre\u2010screenings for aggregation inhibitors, further structural analyses as well as protein\u2013protein interaction studies. Copyright \u00a9 2010 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.1252","type":"journal-article","created":{"date-parts":[[2010,5,27]],"date-time":"2010-05-27T14:05:26Z","timestamp":1274969126000},"page":"358-363","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Synthesis of pathological and nonpathological human exon 1 huntingtin"],"prefix":"10.1002","volume":"16","author":[{"given":"David","family":"Singer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Zauner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maika","family":"Genz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thole","family":"Zuchner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,5,27]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199805000-00001"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90585-E"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81369-0"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80513-9"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.1998.tb00196.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-13-05159.1999"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1409-03.2004"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400250"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1570"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.10.053"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M703682200"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.09.023"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/psc.552"},{"key":"e_1_2_6_15_2","first-page":"90","article-title":"Investigation of the structure of the N\u2010terminal region of prion protein (PrP) via the microwave synthesis of peptide fragments up to 111 amino acids in length","volume":"14","author":"Hill A","year":"2008","journal-title":"J. Pept. 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