{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T11:05:40Z","timestamp":1760267140552},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,11,21]],"date-time":"2011-11-21T00:00:00Z","timestamp":1321833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:p>Several polypeptides aggregate into insoluble amyloid fibrils associated with pathologies such as Alzheimer's disease, Parkinson's disease and type 2 diabetes. Understanding the structural and sequential motifs that drive fibrillisation may assist in the discovery and refinement of effective therapies. Here we investigate the effects of three predicted amyloidogenic regions on the structure of aggregates formed by medin, a poorly characterised polypeptide associated with aortic medial amyloidosis. Solid\u2010state NMR is used to compare the dynamics and sheet packing arrangement of the C\u2010terminal region encompassing residues F<jats:sup>43<\/jats:sup>GSV within full\u2010length medin (Med<jats:sub>1\u201050<\/jats:sub>) and two shorter peptide fragments, Med<jats:sub>30\u201050<\/jats:sub> and Med<jats:sub>42\u201049<\/jats:sub>, lacking specific sequences predicted to be amyloidogenic.<jats:sub>.<\/jats:sub> Results show that all three peptides have different aggregate morphologies, and Med<jats:sub>30\u201050<\/jats:sub> and Med<jats:sub>1\u201050<\/jats:sub> have different sheet packing arrangements and dynamics to Med<jats:sub>42\u201049<\/jats:sub>. These results imply that at least two of the three predicted amyloidogenic regions are required for the formation and elongation of medin fibres observed in the disease state. Copyright \u00a9 2011 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.1418","type":"journal-article","created":{"date-parts":[[2011,11,21]],"date-time":"2011-11-21T06:31:15Z","timestamp":1321857075000},"page":"65-72","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Solid\u2010state NMR reveals differences in the packing arrangements of peptide aggregates derived from the aortic amyloid polypeptide medin"],"prefix":"10.1002","volume":"18","author":[{"given":"Hannah A.","family":"Davies","sequence":"first","affiliation":[{"name":"Institute of Integrative Biology University of Liverpool  Crown Street Liverpool L69 7ZB UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jillian","family":"Madine","sequence":"additional","affiliation":[{"name":"Institute of Integrative Biology University of Liverpool  Crown Street Liverpool L69 7ZB UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Middleton","sequence":"additional","affiliation":[{"name":"Institute of Integrative Biology University of Liverpool  Crown Street Liverpool L69 7ZB UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,11,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02261"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.75.101304.123901"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03680"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05695"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3422"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja802483d"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0370692"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9002666"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(02)75244-2"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.230315097"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.262663499"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi991527v"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja000645z"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0011330"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2003.10.044"},{"key":"e_1_2_5_17_1","first-page":"871","article-title":"Senile aortic amyloid \u2013 evidence for 2 distinct forms of localized deposits","volume":"140","author":"Mucchiano G","year":"1992","journal-title":"Am. 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