{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T19:52:13Z","timestamp":1771617133092,"version":"3.50.1"},"reference-count":60,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,5,2]],"date-time":"2014-05-02T00:00:00Z","timestamp":1398988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","award":["BT\/PR7705\/NNT\/28\/656\/2013"],"award-info":[{"award-number":["BT\/PR7705\/NNT\/28\/656\/2013"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p>Huntington's and eight other neurodegenerative diseases occur because of CAG repeat expansion mutation culminating into an expanded polyglutamine tract in respective protein. In Huntington's disease (HD), a number of CAG repeats beyond normal repeat length (&gt;36) lead to the formation of mutant protein, the proteolytic cleavage of which induces aggregation in polyglutamine length\u2010dependent manner. The neurodegeneration in this disease is linked to aggregation, and its inhibition is a potential approach for therapeutic development. Although peptides and other molecules have been developed for inhibiting aggregation, peptides in general are susceptible to degradation<jats:italic>in vivo<\/jats:italic>conditions. To understand their clinical significance, they also need to be delivered through blood\u2013brain barrier. Here, for the first time, we have synthesized poly\u2010<jats:sc>d<\/jats:sc>,<jats:sc>l<\/jats:sc>\u2010lactide\u2010co\u2010glycolide nanoparticles containing a polyglutamine aggregation inhibitor peptide PGQ<jats:sub>9<\/jats:sub>[P<jats:sup>2<\/jats:sup>], by nanoprecipitation method. This process yielded less than 200\u2009nm spherical nanoparticles with uniform distribution. Characterization studies by infrared spectroscopy\u2010based and HPLC\u2010based assays show the presence of PGQ<jats:sub>9<\/jats:sub>[P<jats:sup>2<\/jats:sup>] in nanoparticles.<jats:italic>In vitro<\/jats:italic>release kinetics demonstrates that nanoparticles release PGQ<jats:sub>9<\/jats:sub>[P<jats:sup>2<\/jats:sup>] by erosion and diffusion processes. When the PGQ<jats:sub>9<\/jats:sub>[P<jats:sup>2<\/jats:sup>]\u2010loaded nanoparticles are incubated with aggregation\u2010prone Q<jats:sub>35<\/jats:sub>P<jats:sub>10<\/jats:sub>peptide, representing<jats:italic>N<\/jats:italic>\u2010terminal part of Huntingtin protein, it arrests the elongation phase of Q<jats:sub>35<\/jats:sub>P<jats:sub>10<\/jats:sub>aggregation. These findings propose the first step toward delivery of a peptide inhibitor against polyglutamine aggregation in HD. Copyright \u00a9 2014 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.2640","type":"journal-article","created":{"date-parts":[[2014,5,2]],"date-time":"2014-05-02T08:06:09Z","timestamp":1399017969000},"page":"630-639","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Biodegradable delivery system containing a peptide inhibitor of polyglutamine aggregation: a step toward therapeutic development in Huntington's disease"],"prefix":"10.1002","volume":"20","author":[{"given":"Abhayraj S.","family":"Joshi","sequence":"first","affiliation":[{"name":"Department of Biological Sciences and Bioengineering Indian Institute of Technology Kanpur Uttar Pradesh 208016 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashwani Kumar","family":"Thakur","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences and Bioengineering Indian Institute of Technology Kanpur Uttar Pradesh 208016 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,5,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/3527600906.mcb.200500027"},{"key":"e_1_2_7_3_1","volume-title":"Huntington's Disease","author":"Bates G","year":"2002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.04.026"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M804813200"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00041.2009"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1059581"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq087"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.292.5521.1552"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-5-40"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.252523899"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0237018100"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1570"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2011.12.010"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2011.12.011"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddp467"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-1238fje"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.110138997"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm985"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2005.05.006"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408936102"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2008.06.015"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2011.11.010"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/C2CS35265F"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2012.01.043"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2009.09.001"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.10.053"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi011772q"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2011.01.001"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf070337l"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppsc.19920090117"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2006.12.075"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/pol.1966.150040626"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018970030327"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.360370404"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-010-9185-1"},{"key":"e_1_2_7_37_1","doi-asserted-by":"crossref","DOI":"10.1017\/CBO9780511806384","volume-title":"Exp. 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