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Antimicrobial peptides are considered potential alternatives to conventional antibiotics. The skin of Anurans (frogs and toads) amphibians is an extraordinarily rich source of antimicrobial peptides. CPF\u2010C1 is a typical cationic antimicrobial peptide that was originally isolated from the tetraploid frog <jats:italic>Xenopus clivii.<\/jats:italic> Our results showed that CPF\u2010C1 has potent antimicrobial activity against both sensitive and multidrug\u2010resistant bacteria. It disrupted the outer and inner membranes of bacterial cells. CPF\u2010C1 induced both propidium iodide uptake into the bacterial cell and the leakage of calcein from large liposome vesicles, which suggests a mode of action that involves membrane disturbance. Scanning electron microscopy and transmission electron microscopy verified the morphologic changes of CPF\u2010C1\u2010treated bacterial cells and large liposome vesicles. The membrane\u2010dependent mode of action signifies that the CPF\u2010C1 peptide functions freely and without regard to conventional resistant mechanisms. Additionally, it is difficult for bacteria to develop resistance against CPF\u2010C1 under this action mode. Other studies indicated that CPF\u2010C1 had low cytotoxicity against mammalian cell. In conclusion, considering the increase in multidrug\u2010resistant bacterial infections, CPF\u2010C1 may offer a new strategy that can be considered a potential therapeutic agent for the treatment of diseases caused by multidrug\u2010resistant bacteria. Copyright \u00a9 2014 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.2679","type":"journal-article","created":{"date-parts":[[2014,8,7]],"date-time":"2014-08-07T01:34:30Z","timestamp":1407375270000},"page":"876-884","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Antimicrobial activities and membrane\u2010active mechanism of CPF\u2010C1 against multidrug\u2010resistant bacteria, a novel antimicrobial peptide derived from skin secretions of the tetraploid frog <i>Xenopus clivii<\/i>"],"prefix":"10.1002","volume":"20","author":[{"given":"Junqiu","family":"Xie","sequence":"first","affiliation":[{"name":"Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences Lanzhou University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanmei","family":"Gou","sequence":"additional","affiliation":[{"name":"Key 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