{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:45:00Z","timestamp":1791308700238,"version":"4.3.1"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T00:00:00Z","timestamp":1418947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000272","name":"National Institute for Health Research","doi-asserted-by":"publisher","award":["P30 GM 103410"],"award-info":[{"award-number":["P30 GM 103410"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health Research","doi-asserted-by":"publisher","award":["P01 NS 065719"],"award-info":[{"award-number":["P01 NS 065719"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:p>The JC polyomavirus (JCPyV) infects approximately 50% of the human population. In healthy individuals, the infection remains dormant and asymptomatic, but in immuno\u2010suppressed patients, it can cause progressive multifocal leukoencephalopathy (PML), a potentially fatal demyelinating disease. Currently, there are no drugs against JCPyV infection nor for the treatment of PML. Here, we report the development of small\u2010molecule inhibitors of JCPyV that target the initial interaction between the virus and host cell and thereby block viral entry. Utilizing a combination of computational and NMR\u2010based screening techniques, we target the LSTc tetrasaccharide binding site within the VP1 pentameric coat protein of JCPyV. Four of the compounds from the screen effectively block viral infection in our <jats:italic>in vitro<\/jats:italic> assays using SVG\u2010A cells. For the most potent compound, we used saturation transfer difference NMR to determine the mode of binding to purified pentamers of JCPyV VP1. Collectively, these results demonstrate the viability of this class of compounds for eventual development of JCPyV\u2010antiviral therapeutics. Copyright \u00a9 2014 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.2731","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T10:51:18Z","timestamp":1418986278000},"page":"236-242","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Small\u2010molecule inhibitors of JC polyomavirus infection"],"prefix":"10.1002","volume":"21","author":[{"given":"Achani","family":"Yatawara","sequence":"first","affiliation":[{"name":"Department of Chemistry Dartmouth College  Hanover NH 03755 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Gaidos","sequence":"additional","affiliation":[{"name":"Department of Chemistry Dartmouth College  Hanover NH 03755 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chamila N.","family":"Rupasinghe","sequence":"additional","affiliation":[{"name":"Department of Chemistry Dartmouth College  Hanover NH 03755 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bethany A.","family":"O'Hara","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Cell Biology, and Biochemistry Brown University  Providence RI 02912 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Pellegrini","sequence":"additional","affiliation":[{"name":"Department of Chemistry Dartmouth College  Hanover NH 03755 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter J.","family":"Atwood","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Cell Biology, and Biochemistry Brown University  Providence RI 02912 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale F.","family":"Mierke","sequence":"additional","affiliation":[{"name":"Department of Chemistry Dartmouth College  Hanover NH 03755 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10450"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000363"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/597126"},{"key":"e_1_2_7_5_1","first-page":"71","article-title":"Molecular biology and pathogenesis of human polyomavirus infections","volume":"94","author":"Dorries K","year":"1998","journal-title":"Dev. 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