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It is also essential for biochemical pathways responsible for DNA synthesis and maintenance and for the generation of new red blood cells. Cellular trafficking of FA and folate is based on its high\u2010affinity binding to cognate folate receptor, a protein commonly expressed in several human cancers. Thus, folate conjugates of drugs, plasmids, biosensors, contrast, and radiodiagnostic imaging agents have been used for assisted delivery in folate receptor\u2010positive cancer cells, via endocytosis pathways. This report describes morphologies of soft structures from a fully characterized FA\u2013dipeptide conjugate and detailed mechanistic studies of its cancer cell uptake, as tracked by the inherent fluorescence of the conjugate. 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