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Alpha\u2010helical antimicrobial peptides (AMPs) may be promising candidates in this respect due to their potent and broad\u2010spectrum antimicrobial activity, their low tendency to elicit resistance and possible retention of efficacy in the immobilized state.<\/jats:p><jats:p>The aim of this study was to evaluate the potential of five different helical AMPs, the cathelicidins BMAP\u201027 and BMAP\u201028, their (1\u201318) fragments and the rationally designed, artificial P19(9\/G7) peptide, for the prevention of orthopaedic implant infections. Peptides were effective at micromolar concentrations against 22 <jats:italic>Staphylococcus<\/jats:italic> and <jats:italic>Streptococcus<\/jats:italic> isolates from orthopaedic infections, while only BMAP\u201028 and to a lesser extent BMAP\u201027 were active against <jats:italic>Enterococcus faecalis<\/jats:italic>. Peptides in solution showed activities comparable to those of cefazolin and linezolid, on a molar basis, and also a variable capacity to neutralize bacterial lipopolysaccharide, while devoid of adverse effects on MG\u201063 osteoblast cells at concentrations corresponding to the MIC. The (1\u201318) BMAP fragments and P19(9\/G7) were selected for further examination, based on better selectivity indices, and showed effectiveness in the presence of hyaluronic acid and in synovial fluid, while human serum affected their activity to variable extents, with BMAP\u201027(1\u201318) best retaining activity. This peptide was immobilized on streptavidin\u2013resin beads and retained activity against reference <jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus epidermidis<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus aureus<\/jats:italic><\/jats:styled-content> strains, with negligible toxicity towards osteoblasts, underlining its potential for the development of infection\u2010resistant biomaterials for orthopaedic application. Copyright \u00a9 2017 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.3026","type":"journal-article","created":{"date-parts":[[2017,7,14]],"date-time":"2017-07-14T09:38:27Z","timestamp":1500025107000},"page":"777-789","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of free or anchored antimicrobial peptides as candidates for the prevention of orthopaedic device\u2010related infections"],"prefix":"10.1002","volume":"23","author":[{"given":"Francesca","family":"D'Este","sequence":"first","affiliation":[{"name":"Department of Medicine University of Udine  P.le Kolbe 4 33100 Udine Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debora","family":"Oro","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Udine  P.le Kolbe 4 33100 Udine Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Boix\u2010Lemonche","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Udine  P.le Kolbe 4 33100 Udine Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Tossi","sequence":"additional","affiliation":[{"name":"Department of Life Sciences University of Trieste  Via Giorgieri 5 34127 Trieste Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0437-802X","authenticated-orcid":false,"given":"Barbara","family":"Skerlavaj","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Udine  P.le Kolbe 4 33100 Udine Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.b1773"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arth.2013.01.012"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00111-13"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2016.09.019"},{"key":"e_1_2_7_6_1","first-page":"1","volume-title":"Surveillance of Surgical Site Infections in Europe 2010\u20132011","author":"ECDC. 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