{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:56:48Z","timestamp":1761490608829,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"8-9","license":[{"start":{"date-parts":[[2018,7,16]],"date-time":"2018-07-16T00:00:00Z","timestamp":1531699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["91753205"],"award-info":[{"award-number":["91753205"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:p>The Piezo channel is a versatile mechanosensitive cation channel that mediates tactile, vascular development, and proprioception. GsMTx4 is the only reported inhibitor specifically targeting Piezo channels. Although the sequence of GsMTx4 is reported, the crystal structure of GsMTx4 is still unknown. Here, we achieved the two\u2010segment synthesis of GsMTx4 and its enantiomer, enGsMTx4, through hydrazide based Native Chemical Ligation, and analyzed the crystal structure of GsMTx4 through the racemic crystallization technology. By analyzing the structure, we found that there is a hydrophobic patch surrounded by aromatic residues and charged residues.<\/jats:p>","DOI":"10.1002\/psc.3112","type":"journal-article","created":{"date-parts":[[2018,7,16]],"date-time":"2018-07-16T08:04:21Z","timestamp":1531728261000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Racemic crystal structures of peptide toxins, GsMTx4 prepared by protein total synthesis"],"prefix":"10.1002","volume":"24","author":[{"given":"Qian","family":"Qu","sequence":"first","affiliation":[{"name":"Department of Chemistry, Tsinghua\u2010Peking Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology Tsinghua University  Beijing 100084 China"},{"name":"School of Biological and Medical Engineering Hefei University of Technology  Anhui Hefei 230009 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tsinghua\u2010Peking Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology Tsinghua University  Beijing 100084 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2161-2482","authenticated-orcid":false,"given":"Yi\u2010Ming","family":"Li","sequence":"additional","affiliation":[{"name":"School of Biological and Medical Engineering Hefei University of Technology  Anhui Hefei 230009 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2595"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.08.032"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1193270"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10812"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1221400110"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2899"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1219777110"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2014.03.015"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25743"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25453"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.115.5.583"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202715200"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(03)00141-7"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1063-5823(06)59004-0"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2006.09.030"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201100996"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201203843"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.152"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201310010"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201500051"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja500222u"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2517"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.6b04031"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.6b09545"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.2868"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.2863"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2014.03.022"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02743"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.468"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja8077973"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.127"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja8013538"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biophys-050511-102333"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja311408y"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200463040"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1422387112"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1210483109"},{"issue":"37","key":"e_1_2_6_39_1","first-page":"13974","article-title":"Engineering highly potent and selective microproteins against Nav1.7 sodium channel for treatment of pain","volume":"291","author":"A1 S","year":"2016","journal-title":"J Biol Chem"},{"issue":"2","key":"e_1_2_6_40_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive python\u2010based system for macromolecular structure solution","volume":"66","author":"PD1 A","year":"2010","journal-title":"Acta Crystallogr D Biol Crystallogr"}],"container-title":["Journal of Peptide Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpsc.3112","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/psc.3112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T07:49:10Z","timestamp":1695023350000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/psc.3112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,16]]},"references-count":39,"journal-issue":{"issue":"8-9","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["10.1002\/psc.3112"],"URL":"https:\/\/doi.org\/10.1002\/psc.3112","archive":["Portico"],"relation":{},"ISSN":["1075-2617","1099-1387"],"issn-type":[{"type":"print","value":"1075-2617"},{"type":"electronic","value":"1099-1387"}],"subject":[],"published":{"date-parts":[[2018,7,16]]},"assertion":[{"value":"2018-04-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e3112"}}