{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T11:14:41Z","timestamp":1773746081969,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,11,4]],"date-time":"2018-11-04T00:00:00Z","timestamp":1541289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:p>Scorpion venom contains various bioactive peptides. Among them, peptides having two different structural domains constitute a toxin family known as \u03b2\u2010KTx or scorpine\u2010like peptides. These peptides consist of an \u03b1\u2010helical structure in the N\u2010terminal region and a cysteine\u2010stabilized structure in the C\u2010terminal region. This unique structure of \u03b2\u2010KTx peptides contributes to their diverse biological functions, but the importance of each domain for their activities is not fully understood. LaIT2 is a \u03b2\u2010KTx peptide isolated from the venom of the scorpion <jats:italic>Liocheles australasiae<\/jats:italic>, which shows both insecticidal and antimicrobial activities. In this study, we chemically synthesized full\u2010length LaIT2 using a native chemical ligation technique as well as its N\u2010terminally or C\u2010terminally truncated single\u2010domain analogs to evaluate structural factors important for the activities. Biological evaluation of these peptides revealed that the N\u2010terminal \u03b1\u2010helical domain of LaIT2 is essential for the expression of both insecticidal and antibacterial activities. This suggests that the disruption of membrane structures largely accounts for the biological activities of LaIT2.<\/jats:p>","DOI":"10.1002\/psc.3133","type":"journal-article","created":{"date-parts":[[2018,11,5]],"date-time":"2018-11-05T06:59:36Z","timestamp":1541401176000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Chemical synthesis of a two\u2010domain scorpion toxin LaIT2 and its single\u2010domain analogs to elucidate structural factors important for insecticidal and antimicrobial activities"],"prefix":"10.1002","volume":"24","author":[{"given":"Hironori","family":"Juichi","sequence":"first","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryo","family":"Ando","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of 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