{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T10:44:21Z","timestamp":1785321861559,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,1,6]],"date-time":"2021-01-06T00:00:00Z","timestamp":1609891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:p>The red tree frog <jats:italic>Litoria rubella<\/jats:italic> from Australia has been studied for several decades showing that their dorsal skin glands secrete a number of small peptides containing a Pro\u2013Trp sequence, known as tryptophyllin L peptides. Although peptides from many genera of Australian frogs have been reported to possess a variety of biological activities, the bioactivities of this peptide family have remained to be discovered. In this study, we investigated the antioxidant potency of a number of tryptophyllin L peptides for the first time using a joint statistical and experimental approach in which predictions based on Gaussian three\u2010dimensional quantitative structure\u2013activity relationship (3D\u2010QSAR) models were employed to guide an in vitro experimental investigation. Two tryptophyllin tripeptides P\u2013W\u2013L (OH) and P\u2013W\u2013L (NH<jats:sub>2<\/jats:sub>) were predicted to have the Trolox equivalent antioxidant capacity (TEAC) values of 0.80 and 0.87 \u03bcM Trolox\/\u03bcM peptide, respectively. With those promising results, antioxidant capabilities of five tryptophyllin L peptides with the common core Pro\u2013Trp\u2013Leu were synthesized and subjected to 1,1\u2010diphenyl\u20102\u2010picrylhydrazyl (DPPH), ferric reducing ability of plasma (FRAP) and 2,2\u2032\u2010azino\u2010bis(3\u2010ethylbenzothiazoline\u20106\u2010sulphonic acid) radical cation (ABTS\u02d9<jats:sup>+<\/jats:sup>) radical scavenging assays. The tests indicated that all the tested tryptophyllin L peptides, noticeably S\u2013P\u2013W\u2013L (OH) and F\u2013P\u2013W\u2013L (NH<jats:sub>2<\/jats:sub>), are strong ABTS\u02d9<jats:sup>+<\/jats:sup> radical scavengers and moderate scavengers in the other two assays. The results, thus, suggested that the tryptophyllin L peptides are likely to be a part of the skin antioxidant system helping the frog to cope with drastic change in oxygen exposure and humidity, as they inhabit over a large area of Australia with a wide climate variation.<\/jats:p>","DOI":"10.1002\/psc.3295","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T09:35:24Z","timestamp":1610271324000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Antioxidant activities of major tryptophyllin L peptides: A joint investigation of Gaussian\u2010based 3D\u2010QSAR and radical scavenging experiments"],"prefix":"10.1002","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4792-6540","authenticated-orcid":false,"given":"Thi Thanh Nha","family":"Tran","sequence":"first","affiliation":[{"name":"Faculty of Chemical Engineering Industrial University of HoChiMinh City  12 Nguyen Van Bao Ho Chi Minh 700000 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinh Phien","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Environment Vietnam\u2010Russia Tropical Centre  63 Nguyen Van Huyen, Nghia Do, Cau Giay Ha Noi 11307 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Van Cuong","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Chemical Engineering Industrial University of HoChiMinh City  12 Nguyen Van Bao Ho Chi Minh 700000 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Dieu Thuan","family":"Tran","sequence":"additional","affiliation":[{"name":"Faculty of Chemical Engineering Industrial University of HoChiMinh City  12 Nguyen Van Bao Ho Chi Minh 700000 Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Thu Thuy","family":"Bui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Hamilton","family":"Bowie","sequence":"additional","affiliation":[{"name":"Faculty of Science The University of Adelaide  Adelaide South Australia 5005 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(00)88947-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0082(93)90027-P"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3724\/SP.J.1245.2013.00062"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2003.09.004"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-14-110"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2007.05.005"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(99)00133-6"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2017.11.001"},{"key":"e_1_2_6_10_1","first-page":"1","article-title":"Rich diversity and potency of skin antioxidant peptides revealed a novel molecular basis for high\u2010altitude adaptation of amphibians","volume":"6","author":"Yang X","year":"2016","journal-title":"Sci Rep"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24020347"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2010.01.036"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M800297-MCP200"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2006.02.030"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0199295"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2004.03.006"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012369442-3\/50046-5"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/b512118n"},{"key":"e_1_2_6_19_1","volume-title":"A Field Guide to Australian Frogs","author":"Barker J","year":"1995"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19960115)10:1<92::AID-RCM443>3.0.CO;2-3"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1071\/CH9960955"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1071\/CH9942099"},{"key":"e_1_2_6_23_1","unstructured":"Nha TranTT.Structural and mechanistic studies of post\u2010translationally modified peptides and proteins. 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