{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:05:47Z","timestamp":1760295947310},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2001,8,31]],"date-time":"2001-08-31T00:00:00Z","timestamp":999216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2001,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The conjugation of synthetic peptides to carrier proteins is a widely used method for immunological studies. Different coupling agents have been described to form the conjugate with carrier proteins. In this paper, we demonstrate that the antibody response toward V3\u2010based synthetic MAPs derived from HIV\u20101, JY1 isolate, conjugated to two different carrier proteins using either <jats:italic>m<\/jats:italic>\u2010maleimidobenzoyl\u2010<jats:italic>N<\/jats:italic>\u2010hydroxysuccinimide ester (MBS) or \u03b2\u2010maleimidopropionic acid <jats:italic>N<\/jats:italic>\u2010hydroxysuccinimide ester (MPS), or succinic anhydride (SA) show different behaviors. An excellent anti\u2010JY1 response without a strong response to the coupling agent is observed in the case of succinic anhydride spacer. In contrast, MBS produces total abrogation of the antibody response with a high response toward the coupling agent. Copyright \u00a9 2001 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.336","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T22:31:19Z","timestamp":1030314679000},"page":"511-518","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Study of different coupling agents in the conjugation of a V3\u2010based synthetic MAP to carrier proteins"],"prefix":"10.1002","volume":"7","author":[{"given":"Luis J.","family":"Cruz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrique","family":"Iglesias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio C.","family":"Aguilar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diogenes","family":"Quintana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hilda E.","family":"Garay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Duarte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osvaldo","family":"Reyes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,8,31]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(69)90177-3"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(80)70047-2"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a131053"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.144.3624.1344"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(80)70046-0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1002\/hlca.19750580224","article-title":"Preparation and some properties of meleimido acids and maleoyl derivatives of peptides","volume":"58","author":"Keller O","year":"1975","journal-title":"Helv. 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