{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:42:42Z","timestamp":1793421762067,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T00:00:00Z","timestamp":1630368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2019M652307 and 2020T130332"],"award-info":[{"award-number":["2019M652307 and 2020T130332"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["T130332"],"award-info":[{"award-number":["T130332"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["M652307"],"award-info":[{"award-number":["M652307"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017445","name":"Natural Science Fund for Distinguished Young Scholars of Shandong Province","doi-asserted-by":"publisher","award":["ZR2020QH100 for Y. L. and ZR2019BH045"],"award-info":[{"award-number":["ZR2020QH100 for Y. L. and ZR2019BH045"]}],"id":[{"id":"10.13039\/100017445","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017445","name":"Natural Science Fund for Distinguished Young Scholars of Shandong Province","doi-asserted-by":"publisher","award":["ZR2019BH045"],"award-info":[{"award-number":["ZR2019BH045"]}],"id":[{"id":"10.13039\/100017445","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017445","name":"Natural Science Fund for Distinguished Young Scholars of Shandong Province","doi-asserted-by":"publisher","award":["ZR2020QH100"],"award-info":[{"award-number":["ZR2020QH100"]}],"id":[{"id":"10.13039\/100017445","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["1753205, 81621002, and 21621003, 22077002, 2180700"],"award-info":[{"award-number":["1753205, 81621002, and 21621003, 22077002, 2180700"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82003647"],"award-info":[{"award-number":["82003647"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21807063"],"award-info":[{"award-number":["21807063"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21621003"],"award-info":[{"award-number":["21621003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81621002"],"award-info":[{"award-number":["81621002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["91753205"],"award-info":[{"award-number":["91753205"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:p>Long\u2010chain scorpion toxin AaH\u2010II isolated from <jats:italic>Androctonus australis Hector<\/jats:italic> can selectively inhibit mammalian voltage\u2010gated sodium ion channel Na<jats:sub>v<\/jats:sub>1.7 responsible for pain sensation. Efficient chemical synthesis of AaH\u2010II and its derivatives is beneficial to the study of the function and mechanism of Na<jats:sub>v<\/jats:sub>1.7 and the development of potential peptide inhibitors. Herein, we compared three different strategies, namely, direct solid\u2010phase peptide synthesis, hydrazide\u2010based two\u2010segment native chemical ligation, and hydrazide\u2010based three\u2010segment native chemical ligation for the synthesis of AaH\u2010II. The hydrazide\u2010based two\u2010segment native chemical ligation affords the target toxin with the optimal efficiency, which provides a practically robust procedure for the preparation of tool molecules derived from AaH\u2010II to study the biological functions and modulation of Na<jats:sub>v<\/jats:sub>1.7. Our work highlights the importance of selecting suitable segment condensation approach in the chemical synthesis of protein toxins.<\/jats:p>","DOI":"10.1002\/psc.3365","type":"journal-article","created":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:10:07Z","timestamp":1630455007000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of different strategies towards the chemical synthesis of long\u2010chain scorpion toxin AaH\u2010II"],"prefix":"10.1002","volume":"28","author":[{"given":"Yu\u2010Lei","family":"Li","sequence":"first","affiliation":[{"name":"Tsinghua\u2010Peking Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Department of Chemistry Tsinghua University  Beijing China"},{"name":"Department of Pharmacology, School of Pharmacy Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Qu","sequence":"additional","affiliation":[{"name":"Tsinghua\u2010Peking Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Department of Chemistry Tsinghua University  Beijing China"},{"name":"Department of Health Sciences, Institutes of Physical Science and Information Technology Anhui University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0015-5964","authenticated-orcid":false,"given":"Yun\u2010Kun","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Pharmacy Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Liu","sequence":"additional","affiliation":[{"name":"Tsinghua\u2010Peking Center for Life Sciences, Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Department of Chemistry Tsinghua University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Pharmacy Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yani","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, School of Pharmacy Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4149-4062","authenticated-orcid":false,"given":"Ge\u2010Min","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, Institutes of Physical Science and Information Technology Anhui University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2006.09.023"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.199500391"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.701"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2006.09.022"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2012.03.024"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr4003068"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi300776g"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01710-4"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402048200"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00625.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep14973"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20030688"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1994.1270"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2005.01.008"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav8573"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2014.11.233"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11426-019-9513-2"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.315382"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2002.03065.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb14735.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)47295-5"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja8042036"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja8077973"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.14.6404"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201408078"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201404438"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201603420"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb400012k"},{"key":"e_1_2_6_30_1","first-page":"9","article-title":"Structural insights into human acid\u2010sensing ion channel 1a inhibition by snake toxin mambalgin1","author":"Sun D","year":"2020","journal-title":"Elife"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201209523"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201500051"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7973629"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201100996"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201203843"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.152"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.582"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0SC06285E"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr400401e"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.3112"}],"container-title":["Journal of Peptide Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/psc.3365","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/psc.3365","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/psc.3365","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T06:54:19Z","timestamp":1693205659000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/psc.3365"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,31]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1002\/psc.3365"],"URL":"https:\/\/doi.org\/10.1002\/psc.3365","archive":["Portico"],"relation":{},"ISSN":["1075-2617","1099-1387"],"issn-type":[{"value":"1075-2617","type":"print"},{"value":"1099-1387","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,31]]},"assertion":[{"value":"2021-06-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e3365"}}