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The antimicrobial peptide analogue SAMP\u2010A4\u2010C8 (<jats:italic>n<\/jats:italic>\u2010octanoic\u2010VRLLRRRI) with high antimicrobial activity was found in our lab. We speculate that it may kill pathogens by some lethal mechanism of action. In the present investigation, the microbicidal activities of SAMP\u2010A4\u2010C8 and its mechanism of action were investigated. The results demonstrated that SAMP\u2010A4\u2010C8 had lethal activities against <jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus aureus<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>Candida albicans<\/jats:italic><\/jats:styled-content> by cell disruption. Based on its microbicidal activities, we believe that it is worth further research for its potential as drug candidate. The results showed that SAMP\u2010A4\u2010C8, with low propensity to induce the resistance of <jats:styled-content style=\"fixed-case\"><jats:italic>S. aureus<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content>, could kill the persister cells of <jats:styled-content style=\"fixed-case\"><jats:italic>S. aureus<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content>, exhibited biofilm forming inhibition activity and preformed biofilm eradication ability against <jats:styled-content style=\"fixed-case\"><jats:italic>S. aureus<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>C. albicans<\/jats:italic><\/jats:styled-content>, and displayed therapeutic potential on pneumonia in <jats:styled-content style=\"fixed-case\"><jats:italic>S. aureus<\/jats:italic><\/jats:styled-content>\u2010infected mice by reducing lung inflammation. The present study provided a promising drug candidate in the war against multidrug resistance.<\/jats:p>","DOI":"10.1002\/psc.3497","type":"journal-article","created":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T01:23:11Z","timestamp":1682299391000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Activities of a broad\u2010spectrum antimicrobial peptide analogue SAMP\u2010A4\u2010C8 and its combat against pneumonia in <scp><i>Staphylococcus aureus\u2010<\/i><\/scp>infected mice"],"prefix":"10.1002","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4824-0930","authenticated-orcid":false,"given":"Ruifang","family":"Li","sequence":"first","affiliation":[{"name":"College of Biological Engineering Henan University of Technology  Zhengzhou China"},{"name":"Key Laboratory of Functional Molecules for Biomedical Research  Zhengzhou 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