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Developing an effective vaccine targeting ST presents challenges due to its potent enterotoxicity, non\u2010immunogenicity, and the risk of autoimmune reaction stemming from its structural similarity to the human endogenous ligands, guanylin, and uroguanylin. This study aimed to assess a novel synthetic vaccine carrier platform employing a single chemical coupling step for making human ST (STh) immunogenic. Specifically, the method involved cross\u2010linking STh to an 8\u2010arm N\u2010hydroxysuccinimide (NHS) ester\u2010activated PEG cross\u2010linker. A conjugate of STh with 8\u2010arm structure was prepared, and its formation was confirmed through immunoblotting analysis. The impact of conjugation on STh epitopes was assessed using ELISAs with polyclonal and monoclonal antibodies targeting various epitopes of STh. Immunization of mice with the conjugate induced the production of anti\u2010STh antibodies, exhibiting neutralizing activity against STh.<\/jats:p>","DOI":"10.1002\/psc.3647","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T06:08:43Z","timestamp":1722578923000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Coupling enterotoxigenic <scp><i>Escherichia coli<\/i><\/scp> heat\u2010stable peptide toxin with 8\u2010arm PEG enhances immunogenicity"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9522-5461","authenticated-orcid":false,"given":"Ephrem Debebe","family":"Zegeye","sequence":"first","affiliation":[{"name":"Marine Biotechnology NORCE Norwegian Research Centre  Bergen Norway"},{"name":"Department of Paraclinical Sciences, Faculty of Veterinary Medicine Norwegian University of Life Sciences (NMBU)  \u00c5s 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