{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:53:59Z","timestamp":1786978439626,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,3]],"date-time":"2024-11-03T00:00:00Z","timestamp":1730592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:p>\n                    Scorpion venom contains linear peptides without disulfide bonds in addition to peptides with disulfide bonds. Many such linear peptides have an amphiphilic \u03b1\u2010helical structure, often with antimicrobial activity and can be classified into three groups based on their molecular size. Among them, long\u2010chain antimicrobial peptides consisting of more than 40 residues have not been thoroughly studied due to the difficulty of synthesizing them. We have previously reported a transcriptome analysis of the venom gland of\n                    <jats:italic>Liocheles australasiae<\/jats:italic>\n                    that revealed precursor sequences of long\u2010chain antimicrobial peptides. In the study reported here, we identified the mature structure of one such long\u2010chain antimicrobial peptide, LaCT1, which we synthesized using chemical ligation to confirm its structure and evaluate its biological activities. The result showed that LaCT1 exhibited significant antimicrobial activity. In addition, we identified its partial peptides consisting of an N\u2010 or C\u2010terminal region, which may be generated by enzymatic cleavage in the venom. Among them, only the peptide containing the N\u2010terminal half region was active. LaCT1 also not only showed insecticidal activity but also synergistically enhanced the effects of another insecticidal peptide identified in\n                    <jats:italic>L. australasiae<\/jats:italic>\n                    venom as well. These results provide insights into the role of antimicrobial peptides in scorpion venom.\n                  <\/jats:p>","DOI":"10.1002\/psc.3661","type":"journal-article","created":{"date-parts":[[2024,11,3]],"date-time":"2024-11-03T20:50:21Z","timestamp":1730667021000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification and synthesis of a long\u2010chain antimicrobial peptide from the venom of the\n                    <i>Liocheles australasiae<\/i>\n                    scorpion"],"prefix":"10.1002","volume":"31","author":[{"given":"Masahiro","family":"Miyashita","sequence":"first","affiliation":[{"name":"Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoichi","family":"Sakai","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture Kyoto University  Kyoto 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