{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T11:24:27Z","timestamp":1778671467514,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,2,3]],"date-time":"2003-02-03T00:00:00Z","timestamp":1044230400000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A toxin with four disulfide bridges from <jats:italic>Tityus serrulatus<\/jats:italic> venom was able to compete with <jats:sup>125<\/jats:sup>I\u2010kaliotoxin on rat brain synaptosomal preparations, with an IC<jats:sub>50<\/jats:sub> of 46 n<jats:sc>M<\/jats:sc>. The obtained amino acid sequence and molecular mass are identical to the previously described butantoxin. Enzymatic cleavages in the native peptide followed by mass spectrometry peptide mapping analysis were used to determine the disulfide bridge pattern of \u03b1\u2010KTx<jats:sub>12\u22121<\/jats:sub>. Also, after the cleavage of the first six <jats:italic>N<\/jats:italic>\u2010terminal residues, including the unusual disulfide bridge which forms an <jats:italic>N<\/jats:italic>\u2010terminus ring, the potency of the cleaved peptide was found to decrease about 100 fold compared with the native protein. Copyright \u00a9 2003 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.440","type":"journal-article","created":{"date-parts":[[2003,2,24]],"date-time":"2003-02-24T14:02:51Z","timestamp":1046095371000},"page":"132-140","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Covalent structure and some pharmacological features of native and cleaved \u03b1\u2010KTx<sub>12\u22121<\/sub>, a four disulfide\u2010bridged toxin from <i>Tityus serrulatus<\/i> venom"],"prefix":"10.1002","volume":"9","author":[{"given":"A. M. C.","family":"Pimenta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Mansuelle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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