{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T14:28:57Z","timestamp":1791124137338,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2003,7,4]],"date-time":"2003-07-04T00:00:00Z","timestamp":1057276800000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Hungarian National Research Fund OTKA","award":["T025838 (to SVH)"],"award-info":[{"award-number":["T025838 (to SVH)"]}]},{"name":"Hungarian National Research Fund OTKA","award":["T37916 (to KFM)."],"award-info":[{"award-number":["T37916 (to KFM)."]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["NCRR 01614"],"award-info":[{"award-number":["NCRR 01614"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["RR12961."],"award-info":[{"award-number":["RR12961."]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>According to recent investigations, the <jats:italic>C<\/jats:italic>\u2010terminal domain of the tissue inhibitor of matrix metalloproteinases\u20101 (TIMP\u20101) is responsible for some biological effects that are independent of the enzyme\u2010inhibiting effect of the <jats:italic>N<\/jats:italic>\u2010terminal domain of the molecule. The <jats:italic>C<\/jats:italic>\u2010terminal domain has been prepared for structure\u2013biological activity investigations. After the chemical synthesis and the folding of the linear peptide, LC\u2010MS and MALDI\u2010MS analysis revealed that two isomers with different disulphide bond arrangements were formed. Since more than 30 folding experiments resulted in products with a very similar HPLC\u2010profile, it was concluded that in the absence of the TIMP\u20101 <jats:italic>N<\/jats:italic>\u2010terminal domain no entirely correct folding of the <jats:italic>C<\/jats:italic>\u2010terminal domain occurred. Furthermore, it was observed that, in spite of several purification steps, mercury(II) ions were bound to the 6SH\u2010linear peptide; it was demonstrated\u2014using disulphide bonded TIMP\u20101(Cys<jats:sup>145<\/jats:sup>\u2010Cys<jats:sup>166<\/jats:sup>) as a model\u2014that mercury(II) ions can cause peptide degradation at pH 7.8 as well as in 0.1% trifluoroacetic acid. Copyright \u00a9 2003 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.455","type":"journal-article","created":{"date-parts":[[2003,7,15]],"date-time":"2003-07-15T10:08:40Z","timestamp":1058263720000},"page":"430-441","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Synthesis of the <i>C<\/i>\u2010terminal domain of the tissue inhibitor of metalloproteinases\u20101(TIMP\u20101)"],"prefix":"10.1002","volume":"9","author":[{"given":"J\u00f3zsef","family":"B\u00f3di","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolett","family":"Mihala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Hajnal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin F.","family":"Medzihradszky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helga","family":"S\u00fcli\u2010Vargha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,7,4]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780198502685.001.0001","volume-title":"Matrix Metalloproteinases and TIMPs","author":"Woessner JF","year":"2000"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00247a001"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00157a002"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"18 943","DOI":"10.1016\/S0021-9258(17)32258-5","article-title":"Functional domains of human TIMP\u20101 (tissue inhibitor of metalloproteinases)","volume":"269","author":"Bodden MK","year":"1994","journal-title":"J. Biol. 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Nomenclature for peptide fragment ions (positive ions). Appendix 6. Mass values for amino acid residues in peptides","volume":"193","author":"Biemann K","year":"1990","journal-title":"Methods Enzymol."},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr00050a003"}],"container-title":["Journal of Peptide Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpsc.455","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/psc.455","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T16:30:04Z","timestamp":1700238604000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/psc.455"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":16,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1002\/psc.455"],"URL":"https:\/\/doi.org\/10.1002\/psc.455","archive":["Portico"],"relation":{},"ISSN":["1075-2617","1099-1387"],"issn-type":[{"value":"1075-2617","type":"print"},{"value":"1099-1387","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]},"assertion":[{"value":"2002-10-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-12-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-07-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}