{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T05:50:52Z","timestamp":1763704252884},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,1,21]],"date-time":"2004-01-21T00:00:00Z","timestamp":1074643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Four monoepitopic MAPs (MAP A, B, C and E) and one bis\u2010diepitopic MAP B\u2010E derived from the primary sequence of <jats:italic>Schistosoma mansoni<\/jats:italic> glyceraldehyde 3\u2010phosphate dehydrogenase, previously tested in BALB\/c mice, were examined for their immunogenicity and protective capacity in C57BL\/6 mice. Despite multimerization into MAPs, MAP A and MAP C were poorly immunogenic. In contrast to BALB\/c mice, MAP E was non\u2010immunogenic in C57BL\/6 mice. Peptide B in the form of MAP B or bis\u2010diepitopic MAP B\u2010E elicited immune responses in C57BL\/6 mice that were associated with a significant decrease in worm burden.<\/jats:p><jats:p>The MAPs were prepared by the stepwise solid\u2010phase peptide synthesis using Boc\/Bzl chemistry, successfully purified on the RP\u2010HPLC column and characterized by RP\u2010HPLC, HPCE and MALDI\u2010TOF MS techniques. A general strategy for MAPs purification is discussed here and the purification of MAP B and MAP E is documented in detail. Copyright \u00a9 2004 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.550","type":"journal-article","created":{"date-parts":[[2004,5,24]],"date-time":"2004-05-24T07:03:17Z","timestamp":1085382197000},"page":"350-362","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Peptides and multiple antigen peptides from <i>Schistosoma mansoni<\/i> glyceraldehyde 3\u2010phosphate dehydrogenase: preparation, immunogenicity and immunoprotective capacity in C57BL\/6 mice"],"prefix":"10.1002","volume":"10","author":[{"given":"Pavel","family":"Vepr\u0306ek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Jez\u0306ek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jir\u0306\u00ed","family":"Velek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hatem","family":"Tallima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mona","family":"Montash","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rashika El","family":"Ridi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,1,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1135\/css200104013","article-title":"Immunogenicity and immunoprotective capacity of synthetic peptides from Schistosoma mansoni glyceraldehyde 3\u2010phosphate dehydrogenase","volume":"4","author":"Velek J","year":"2001","journal-title":"Collection Symp. 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