{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:17:08Z","timestamp":1791091028571,"version":"4.1.0"},"reference-count":69,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2004,6,10]],"date-time":"2004-06-10T00:00:00Z","timestamp":1086825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"French CNRS."},{"DOI":"10.13039\/100009759","name":"Universit\u00e9 de la M\u00e9diterran\u00e9e.","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100009759","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Cellpep S.A."}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aah I is a 63\u2010residue \u03b1\u2010toxin isolated from the venom of the <jats:italic>Buthidae<\/jats:italic> scorpion <jats:italic>Androctonus australis hector<\/jats:italic>, which is considered to be the most dangerous species. We report here the first chemical synthesis of Aah I by the solid\u2010phase method, using a Fmoc strategy. The synthetic toxin I (sAah I) was renatured in DMSO\u2010Tris buffer, purified and subjected to thorough analysis and comparison with the natural toxin. The sAah I showed physico\u2010chemical (CD spectrum, molecular mass, HPLC elution), biochemical (amino\u2010acid composition, sequence), immunochemical and pharmacological properties similar to those of the natural toxin. The synthetic toxin was recognized by a conformation\u2010dependent monoclonal anti\u2010Aah I antibody, with an IC<jats:sub>50<\/jats:sub> value close to that for the natural toxin. Following intracerebroventricular injection, the synthetic and the natural toxins were similarly lethal to mice. In voltage\u2010clamp experiments, Na<jats:sub>v<\/jats:sub>1.2 sodium channel inactivation was inhibited by the application of sAah I or of the natural toxin in a similar way. This work describes a simple protocol for the chemical synthesis of a scorpion \u03b1\u2010toxin, making it possible to produce structural analogues in time. Copyright \u00a9 2004 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.582","type":"journal-article","created":{"date-parts":[[2004,6,10]],"date-time":"2004-06-10T16:00:52Z","timestamp":1086883252000},"page":"666-677","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["First chemical synthesis of a scorpion \u03b1\u2010toxin affecting sodium channels: The Aah I toxin of <i>Androctonus australis hector<\/i>"],"prefix":"10.1002","volume":"10","author":[{"given":"Sarrah","family":"M'Barek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziad","family":"Fajloun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Cest\u00e8le","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Devaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Mansuelle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amor","family":"Mosbah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Besma","family":"Jouirou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Mantegazza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jurphaas","family":"van Rietschoten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed El","family":"Ayeb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Rochat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Marc","family":"Sabatier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0328ois","family":"Sampieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,6,10]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(82)90240-9"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(95)00005-7"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(66)90007-9"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/296090a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1016\/S0021-9258(18)45993-5","article-title":"Kaliotoxin, a novel peptidyl inhibitor of neuronal BK\u2010type Ca(2+)\u2010activated K+ channels characterized from Androctonus mauretanicus mauretanicus venom","volume":"267","author":"Crest M","year":"1992","journal-title":"J. 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