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While adjuvants are usually coadministered for nucleic acid and protein vaccines, synthetic peptide antigens afford a more effective opportunity to covalently and regioselectively graft immunostimulatory motifs directly onto the antigen scaffold to yield <jats:italic>self\u2010adjuvanting<\/jats:italic> vaccines. Herein, we explore the synthesis of two tissue\u2010restricted cancer\u2010testis antigens (CTAs); New York oesophageal cell carcinoma 1 (NY\u2010ESO\u20101) and B melanoma antigen 4 (BAGE4), both carrying the toll\u2010like receptor (TLR) agonist, Pam<jats:sub>2<\/jats:sub>Cys. These constructs were evaluated in\u00a0vivo along with a lipid nanoparticle (LNP) preparation of the underexplored BAGE4 melanoma antigen.<\/jats:p>","DOI":"10.1002\/psc.70022","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T08:54:09Z","timestamp":1746521649000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In Vivo Evaluation of Pam<sub>2<\/sub>Cys\u2010Modified Cancer\u2010Testis Antigens as Potential Self\u2010Adjuvanting Cancer Vaccines"],"prefix":"10.1002","volume":"31","author":[{"given":"Salwa","family":"Aljohani","sequence":"first","affiliation":[{"name":"School of Chemistry, University of Nottingham University Park  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5139-8666","authenticated-orcid":false,"given":"Alex\u00a0G.","family":"Edmonds","sequence":"additional","affiliation":[{"name":"School of 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