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Specifically, gelsolin mutations, N184K and D187Y, have been linked to renal amyloidosis and systemic progressive deposition of amyloids, respectively. Understanding how such mutations mitigate gelsolin aggregation and how this process can be prevented through small molecule inhibitors is of interest. Herein, we explored the efficacies of plant\u2010based naturally occurring cytokinin (CK) molecules as aggregation modulators in\u00a0vitro. Using various biophysical methods, such as spectroscopy and microscopy, the aggregation of wild\u2010type gelsolin peptide 184NNGDCFILDL193 and its mutants (N184K, D187Y) was investigated. The mutations significantly promoted aggregation, which is of biological significance. The CK <jats:italic>trans<\/jats:italic>\u2010zeatin (tZ) was a more effective disaggregation promoter compared with kinetin (Kin). The experimentally determined IC<jats:sub>50<\/jats:sub> values were in the 9\u201320\u2009\u03bcM range. The mode of inhibition was identified as direct non\u2010covalent complexation between the CK and the peptides by using mass spectrometry and molecular docking studies. Data show that CKs are promising amyloid modulators, which can be easily translatable to other amyloid systems.<\/jats:p>","DOI":"10.1002\/psc.70057","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T07:36:26Z","timestamp":1757921786000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Plant Hormone Cytokinin as Aggregation Modulator of Gelsolin Amyloidosis"],"prefix":"10.1002","volume":"31","author":[{"given":"Dev","family":"Seneviratne","sequence":"first","affiliation":[{"name":"Environmental and Life Sciences Program Trent University  Peterborough Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3472-9284","authenticated-orcid":false,"given":"Naomi","family":"Stock","sequence":"additional","affiliation":[{"name":"Water Quality Center Trent University  Peterborough Ontario 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