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In soil, it reduces fertility and agricultural productivity. On the other hand, bacteria employ several tolerance mechanisms in response to HM exposure, including the production of siderophores, exopolysaccharides, proteins, and peptides. This study aimed to identify the possible molecular mechanisms involved in Cd(II) tolerance in the <jats:styled-content style=\"fixed-case\"><jats:italic>Delftia lacustris<\/jats:italic><\/jats:styled-content> B11CM strain using nano LC\/MS\u2013MS proteomic analysis, siderophore and exopolysaccharide assays, protein and thiol quantification, and the comparison of protein profiles obtained by SDS\u2010PAGE. The results demonstrated the presence of siderophores, an increase in the production of exopolysaccharides, and thiol\u2010rich compounds. A total of 80 proteins were detected in the presence of cadmium, which are involved in catalytic activity, metalloproteins or proteins that bind to different molecules, and transporters. 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