{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T07:43:36Z","timestamp":1776325416235,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In our pursuit of developing HER2\u2010targeting radiolabeled peptides for detection and therapy of HER2\u2010positive breast cancer, the previously reported rL\u2010A9 peptide had shown promising potential. In the present work, a different variant of rL\u2010A9 was designed by incorporation of peptide\u2010based chelators for\n                    <jats:sup>99m<\/jats:sup>\n                    Tc\u2010labeling. Cysteine\u2010based amino acids (CGGG, CSSG, CEEG) were conjugated at the N\u2010terminus of the peptide and were radiolabeled with [\n                    <jats:sup>99m<\/jats:sup>\n                    Tc]NaTcO\n                    <jats:sub>4<\/jats:sub>\n                    . Of the three radiopeptides, [\n                    <jats:sup>99m<\/jats:sup>\n                    Tc]Tc\u2010CGGG\u2010rL\u2010A9 exhibited high stability, whereas [\n                    <jats:sup>99m<\/jats:sup>\n                    Tc]Tc\u2010CSSG\u2010rL\u2010A9 and [\n                    <jats:sup>99m<\/jats:sup>\n                    Tc]Tc\u2010CEEG\u2010rL\u2010A9 decomposed rapidly. Hence, only CGGG\u2010incorporated radiopeptide was further investigated in\u00a0vitro in human breast carcinoma SKBR3 cells and in\u00a0vivo in tumor xenografted mice. Cell\u2010binding studies demonstrated high binding affinity [K\n                    <jats:sub>d<\/jats:sub>\n                    : 5.82\u2009\u00b1\u20090.92\u2009nM] and specificity (63% reduction in blocking study) of [\n                    <jats:sup>99m<\/jats:sup>\n                    Tc]Tc\u2010CGGG\u2010rL\u2010A9 toward HER2\u2010expressing cells. In\u00a0vivo studies showed moderate tumor uptake (3.56\u2009\u00b1\u20090.81% ID\/g, 1\u2009h) with 82.8% retention at 3\u2009h (3.16\u2009\u00b1\u20090.22% ID\/g). Moderately high tumor\u2010to\u2010background ratios, along with washout from the other non\u2010target organs, were observed. Inhibition studies revealed 52.6% reduction in the tumor activity, signifying high HER2\u2010receptor specificity of the presently studied radiopeptide, [\n                    <jats:sup>99m<\/jats:sup>\n                    Tc]Tc\u2010CGGG\u2010rL\u2010A9.\n                  <\/jats:p>","DOI":"10.1002\/psc.70094","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T11:26:17Z","timestamp":1773919577000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Influence of Peptide\u2010Based Chelator Sequences on HER2\u2010Targeting Radiopeptide"],"prefix":"10.1002","volume":"32","author":[{"given":"Sonal","family":"Gupta","sequence":"first","affiliation":[{"name":"Radiopharmaceuticals Division Bhabha Atomic Research Centre  Mumbai India"},{"name":"KET'S V. G. Vaze College Mumbai University  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manoj","family":"Kumar","sequence":"additional","affiliation":[{"name":"Radiopharmaceuticals Division Bhabha Atomic Research Centre  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishna\u00a0Mohan","family":"Repaka","sequence":"additional","affiliation":[{"name":"Board of Radiation and Isotope Technology  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7968-3574","authenticated-orcid":false,"given":"Amit","family":"Das","sequence":"additional","affiliation":[{"name":"Protein Crystallography Section, Bio\u2010Science Group Bhabha Atomic Research Centre  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajit\u00a0A.","family":"Kengar","sequence":"additional","affiliation":[{"name":"KET'S V. G. Vaze College Mumbai University  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0676-6157","authenticated-orcid":false,"given":"Drishty","family":"Satpati","sequence":"additional","affiliation":[{"name":"Radiopharmaceuticals Division Bhabha Atomic Research Centre  Mumbai India"},{"name":"Homi Bhabha National Institute  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2016.17.4.1609"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcph.765"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23095023"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nucmedbio.2020.05.005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/snuc.2001.26205"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmg.2008.12.013"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr980435t"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2016.11.011"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.0c00334"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.108.053041"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.4024"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.3c00265"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc0300807"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.70064"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ic00111a025"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm900360d"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics13020182"},{"issue":"11","key":"e_1_2_9_19_1","first-page":"1966","article-title":"Optimizing Labeling Conditions for Cysteine\u2010Based Peptides With 99mTc","volume":"27","author":"Sabahnoo H.","year":"2016","journal-title":"Journal of the Brazilian Chemical Society"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232113443"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21256"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21334"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3011.1996.tb00849.x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867327666200312114902"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nucmedbio.2004.02.009"}],"container-title":["Journal of Peptide Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/psc.70094","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/psc.70094","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/psc.70094","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T06:53:37Z","timestamp":1776322417000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/psc.70094"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,19]]},"references-count":24,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/psc.70094"],"URL":"https:\/\/doi.org\/10.1002\/psc.70094","archive":["Portico"],"relation":{},"ISSN":["1075-2617","1099-1387"],"issn-type":[{"value":"1075-2617","type":"print"},{"value":"1099-1387","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,19]]},"assertion":[{"value":"2026-01-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70094"}}