{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T07:14:21Z","timestamp":1779174861276,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":130,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Activating Nrf2 by targeting the Keap1\u2010Nrf2 signaling axis has emerged as a viable therapeutic approach for managing oxidative stress and its associated disorders. This study employed virtual digestion, ADMET profiling, and molecular docking to identify antioxidant peptides derived from\n                    <jats:italic>Ulva prolifera<\/jats:italic>\n                    protein. A novel tetrapeptide (WDGL) was ultimately discovered, demonstrating favorable aqueous solubility, non\u2010toxicity, high intestinal absorption efficiency, and blood\u2013brain barrier permeability. WDGL established four H\u2010bond interactions with some key sites of Keap1 (Arg380, Arg415, Ile416, Leu365). Besides, WDGL reduces ABTS\u00b7\n                    <jats:sup>+<\/jats:sup>\n                    and ferric\u2010tripyridyltriazine (Fe\n                    <jats:sup>3+<\/jats:sup>\n                    \u2010TPTZ) in\u00a0vitro and promotes the expression of GSH\u2010Px while increasing GSH\u2010Px and SOD enzyme activity to eliminate ROS in LPS\u2010treated EA.hy926 cells. Furthermore, this peptide could reduce the content of ROS and ET\u20101 in Ang II\u2010induced EA.hy926 cells. The result suggested that WDGL may alleviate EA.hy926 oxidative damage by activating the Keap1\u2010Nrf2 signaling pathway.\n                  <\/jats:p>","DOI":"10.1002\/psc.70100","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T07:40:48Z","timestamp":1778485248000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and Mechanistic Study of a Novel Keap1\u2010Targeting Antioxidant Peptide From\n                    <scp>\n                      <i>Ulva prolifera<\/i>\n                    <\/scp>\n                    Protein"],"prefix":"10.1002","volume":"32","author":[{"given":"Shaohui","family":"Zhu","sequence":"first","affiliation":[{"name":"Suzhou Chien\u2010shiung Institute of Technology  Suzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanglian","family":"Liu","sequence":"additional","affiliation":[{"name":"Suzhou 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