{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T13:05:42Z","timestamp":1789650342191,"version":"4.0.0"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The synthesis and in\u00a0vitro neuronal growth support activity of a novel series of heptapeptides (\n                    <jats:bold>1<\/jats:bold>\n                    \u2013\n                    <jats:bold>19<\/jats:bold>\n                    ) are reported here. Structural studies of the peptide\n                    <jats:bold>3<\/jats:bold>\n                    (LSAVTFG.DMA) revealed an unexpected dimethylammonium (DMA) salt adduct formation localized at the\n                    <jats:italic>C<\/jats:italic>\n                    \u2010terminal glycine residue. DOSY NMR analysis revealed the presence of two distinct species exhibiting different diffusion coefficients, allowing the differentiation between the peptide and the DMA adduct. Peptide\n                    <jats:bold>3<\/jats:bold>\n                    promoted neural differentiation at 100\u2009\u03bcM concentration. To identify residues crucial for biological activity, we conducted L\u2010 and D\u2010alanine scans with salt and nonsalt form of peptide\n                    <jats:bold>3,<\/jats:bold>\n                    which resulted in the synthesis of 16 peptide analogs. Findings from the alanine scan revealed that the lead peptides L1A.DMA (\n                    <jats:bold>13<\/jats:bold>\n                    ) and S2A.DMA (\n                    <jats:bold>15<\/jats:bold>\n                    ) exhibited significant neural progenitor cells or neural stem cells (NPCs\/NSCs) differentiation activity. These peptides promote neuronal survival, morphology recovery, increase cell proliferation, and preserve neuronal network integrity under OGD\/R and hypoxia\/R insult in an in\u00a0vitro model of ischemic stroke. These results represent the first synthetic approach to new neuromodulatory peptides and deliver important structure\u2013activity relationship (SAR) insights to guide the development of bioactive peptides.\n                  <\/jats:p>","DOI":"10.1002\/psc.70124","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T08:06:38Z","timestamp":1787213198000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Serendipitous Discovery of Short Peptides Dimethylammonium Salt Promoting Neural Differentiation"],"prefix":"10.1002","volume":"32","author":[{"given":"Sadiq Noor","family":"Khan","sequence":"first","affiliation":[{"name":"Third World Center for Science and Technology, H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9906-768X","authenticated-orcid":false,"given":"Maha","family":"Shahid","sequence":"additional","affiliation":[{"name":"H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6758-6730","authenticated-orcid":false,"given":"Shahzad","family":"Nazir","sequence":"additional","affiliation":[{"name":"Third World Center for Science and Technology, H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anila","family":"Bashir","sequence":"additional","affiliation":[{"name":"Third World Center for Science and Technology, H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2604-2765","authenticated-orcid":false,"given":"Sumbla","family":"Sheikh","sequence":"additional","affiliation":[{"name":"Institute for Medical Virology and Epidemiology of Viral Diseases University of T\u00fcbingen  T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9675-842X","authenticated-orcid":false,"given":"Shabana Usman","family":"Simjee","sequence":"additional","affiliation":[{"name":"H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences University of Karachi  Karachi Pakistan"},{"name":"Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3585-4765","authenticated-orcid":false,"given":"Marc","family":"Maresca","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille Univ CNRS, Centrale Marseille, iSm2  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0057-3960","authenticated-orcid":false,"given":"Farzana","family":"Shaheen","sequence":"additional","affiliation":[{"name":"Third World Center for Science and Technology, H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences University of Karachi  Karachi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031851"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2018.05.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3892\/br.2016.630"},{"issue":"4","key":"e_1_2_8_5_1","first-page":"1817","article-title":"Damage to Dopaminergic Neurons by Oxidative Stress in Parkinson's Disease","volume":"41","author":"Guo J. 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