{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:03:44Z","timestamp":1790424224634,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,9,24]],"date-time":"2007-09-24T00:00:00Z","timestamp":1190592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>1<jats:italic>H<\/jats:italic>\u2010Benzotriazolium 1\u2010[<jats:italic>bis<\/jats:italic>(dimethyl\u2010amino)methylene]\u20105\u2010chloro\u2010hexafluorophosphate (1\u2010),3\u2010oxide (HCTU) is a nontoxic, nonirritating and noncorrosive coupling reagent. Seven biologically active peptides (GHRP\u20106, <jats:sup>65\u201374<\/jats:sup>ACP, oxytocin, G\u2010LHRH, <jats:italic>C<\/jats:italic>\u2010peptide, hAmylin<jats:sub>1\u201337<\/jats:sub>, and \u03b2\u2010amyloid<jats:sub>1\u201342<\/jats:sub>) were synthesized with reaction times reduced to deprotection times of 3 min or less and coupling times of 5 min or less using HCTU as the coupling reagent. Expensive coupling reagents or special techniques were not used. Total peptide synthesis times were dramatically reduced by as much as 42.5 h (1.8 days) without reducing the crude peptide purities. It was shown that HCTU can be used as an affordable, efficient coupling reagent for fast Fmoc solid\u2010phase peptide synthesis. 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