{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T04:48:17Z","timestamp":1778820497474,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,12,21]],"date-time":"2007-12-21T00:00:00Z","timestamp":1198195200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Peptide Science"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lycotoxin I and Lycotoxin II are natural anti\u2010microbial peptides that were identified in the venom of the Wolf Spider <jats:italic>Lycosa carolinensis<\/jats:italic>. These peptides were found to be potent growth inhibitors for bacteria (<jats:italic>Escherichia coli<\/jats:italic>) and yeast (<jats:italic>Candida glabrata<\/jats:italic>) at micromolar concentrations. Recently, shortened analogues of LycoI and LycoII have been reported to have decreased haemolytic effects. A shorter Lyco\u2010I analogue studied, LycoI 1\u201315 (H\u2010IWLTALKFLGKHAAK\u2010NH<jats:sub>2<\/jats:sub>), was active only above 10 \u00b5<jats:sc>M<\/jats:sc>, but was also the least haemolytic.<\/jats:p><jats:p>On the basis of these findings, we became interested in obtaining a deeper insight into the membrane activity of LycoI 1\u201315, as this peptide may represent the first major step for the future development of selective, i.e. non\u2010haemolytic, Lycotoxin\u2010based antibiotics.<\/jats:p><jats:p>The interaction of this peptide with liposomes of different composition was studied by microcalorimetry [differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC)] and CD. The results obtained from the calorimetric and spectroscopic techniques were jointly discussed in an attempt to further understand the interaction of this peptide with model membranes. Copyright \u00a9 2007 European Peptide Society and John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/psc.993","type":"journal-article","created":{"date-parts":[[2007,12,21]],"date-time":"2007-12-21T10:18:08Z","timestamp":1198232288000},"page":"528-534","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Membrane structure and interactions of a short Lycotoxin I analogue"],"prefix":"10.1002","volume":"14","author":[{"given":"R.","family":"Ad\u00e3o","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Seixas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Costa","family":"Pessoa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Bastos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,12,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp051572e"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0282(1998)47:6<415::AID-BIP2>3.0.CO;2-D"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200500095"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312260200"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl382"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(04)74108-9"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1098"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413406200"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi035948v"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.10394"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.093013"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389203053027539"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMe020106"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/415389a"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.2174\/1386207053764576"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.4.2059"},{"issue":"1","key":"e_1_2_1_18_2","first-page":"527","article-title":"Leishmanicidal activity of antimicrobial peptides from the wolf spider Lycosa carolinensis","volume":"12","author":"Luque\u2010Ortega JR","year":"2006","journal-title":"J. Pep. Sci"},{"key":"e_1_2_1_19_2","first-page":"41","volume-title":"Physical Properties of Biological Membranes and Their Functional Implications","author":"Blume A","year":"1988"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(99)00204-7"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(86)83497-X"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.1640"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03451.x"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3011.2003.00050.x"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4715"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2004.08.004"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(04)74169-7"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(98)95035-2"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(71)90443-X"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00047-0"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-022X(91)90023-P"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(04)74170-3"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-022X(91)90018-R"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(03)75047-4"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.63.041914"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(01)00382-0"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi980539y"}],"container-title":["Journal of Peptide Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpsc.993","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/psc.993","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T16:51:07Z","timestamp":1694623867000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/psc.993"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12,21]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,4]]}},"alternative-id":["10.1002\/psc.993"],"URL":"https:\/\/doi.org\/10.1002\/psc.993","archive":["Portico"],"relation":{},"ISSN":["1075-2617","1099-1387"],"issn-type":[{"value":"1075-2617","type":"print"},{"value":"1099-1387","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,12,21]]},"assertion":[{"value":"2007-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-11-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-12-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}